Probe:	NIA clone H3140A03
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were hydrolyzed to an average length of 700 bp.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present		2A/A'
TS17: 1st branchial arch maxillary component ectoderm	Present		2A/A'
TS17: 2nd branchial arch	Present		2A/A'
TS17: forebrain	Present		2A/A'
TS17: forelimb bud apical ectodermal ridge	Present		2A/A'
TS17: hindbrain	Present		2A/A'
TS17: hindlimb bud apical ectodermal ridge	Present		2A/A'
TS17: midbrain	Present		2A/A'
TS17: telencephalon	Present	Regionally restricted	2A/A'	Expression is excluded from a region of the anterior telencephalon.
TS17: olfactory epithelium	Present	Regionally restricted	2A/A'	Expression is in the olfactory epithelium surrounding the nasal pits.
TS17: head	Present	Regionally restricted	2A/A'	Expression is in the epithelium of the face.
TS17: fronto-nasal process mesenchyme	Absent		2A/A'

Structure	Level	Pattern	Image	Note
TS19: body wall mesenchyme	Present	Regionally restricted	2B/B'	Expression is in the body wall overlying the pericardial cavity.
TS19: sclerotome condensation	Present	Regionally restricted	2B/B'	Expression is in an intersegmental pattern in the early condensing sclerotome.
TS19: head	Present	Regionally restricted	2B/B'	Expression is in the epithelium of the face.
TS19: mandible primordium	Present		2B/B'
TS19: maxilla primordium	Present		2B/B'
TS19: olfactory epithelium	Present		2B/B'
TS19: oral epithelium	Present		2B/B'
TS19: nose	Present	Regionally restricted	2B/B'	Expression is in the nasal chonchae.
TS19: nasal process	Absent		2B/B'

Structure	Level	Pattern	Image	Note
TS21: head	Present		2C/C'
TS21: lateral ganglionic eminence	Present	Regionally restricted	2C/C'	Expression is in the subventricular zone of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component ectoderm	Present		2D/D'
TS17: 1st branchial arch maxillary component ectoderm	Present		2D/D'
TS17: 2nd branchial arch mesenchyme	Present		2D/D'
TS17: olfactory epithelium	Present		2D/D'
TS17: oral epithelium	Present		2D/D'
TS17: nose	Present	Regionally restricted	2D/D'	Expression is in the nasal chonchae.
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	2D/D'	Expression is in the midline of the mandible.
TS17: 1st branchial arch maxillary component	Present	Regionally restricted	2D/D'	Expression is in the maxilla.
TS17: fronto-nasal process mesenchyme	Absent		2D/D'

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present		2E/E'	Expression is in the epithelia of the maxillary and mandibular prominences.
TS19: 2nd branchial arch mesenchyme	Present		2E/E'
TS19: olfactory epithelium	Present		2E/E'
TS19: oral epithelium	Present		2E/E'
TS19: nose	Present	Regionally restricted	2E/E'	Expression is in the nasal chonchae.
TS19: mandibular process	Present	Regionally restricted	2E/E'	Expression is in the midline of the mandible.
TS19: maxilla primordium	Present	Graded	2E/E'	Expression is stronger close to the zone of fusion between the maxillary and medial nasal prominences.

Structure	Level	Pattern	Image	Note
TS20: mandible primordium	Present	Regionally restricted	2F/F'	Expression is in the midline of the mandible.
TS20: premaxilla	Present	Regionally restricted	2F/F'	Expression is in the midline of the mandible.
TS20: maxilla	Present		2F/F'
TS20: fronto-nasal process mesenchyme	Absent		2F/F'

Structure	Level	Pattern	Image	Note
TS20: palatal shelf	Present		2G/G'
TS20: tongue mesenchyme	Present		2G/G'
TS20: tongue	Present	Regionally restricted	2G/G'	Expression is at the lingual sulcus at the base of the tongue.
TS20: lateral ganglionic eminence	Present	Regionally restricted	2G/G'	Expression is in the subventricular zone of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Present		2H/H'
TS21: tongue mesenchyme	Present		2H/H'
TS21: tongue	Present	Regionally restricted	2H/H'	Expression is at the lingual sulcus at the base of the tongue.
TS21: eye	Present	Regionally restricted	2H/H'	Expression is in the extraocular mesenchyme.
TS21: eyelid	Present		2H/H'	Expression is in the epithelium and mesenchyme.
TS21: lateral ganglionic eminence	Present	Regionally restricted	2H/H'	Expression is in the subventricular zone of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS23: nasal cavity epithelium	Present		2I/I'
TS23: olfactory epithelium	Present		2I/I'
TS23: oral epithelium	Present		2I/I'
TS23: palatal shelf	Present		2I/I'
TS23: turbinate bone primordium	Present		2I/I'
TS23: eye	Present	Regionally restricted	2I/I'	Expression is in the extraocular mesenchyme.
TS23: eyelid	Present		2I/I'	Expression is in the epithelium and mesenchyme.
TS23: lateral ganglionic eminence	Present	Regionally restricted	2I/I'	Expression is in the subventricular zone of the lateral ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS20: eye	Present	Regionally restricted		Expression is in the extraocular mesenchyme and in the epithelium and mesenchyme of the eyelid.