Probe:	NIA clone H3140A03
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural plate	Present	Regionally restricted	1A	Expression is in the lateral edges of the neural plate.
TS13: future brain neural fold	Present		1A
TS13: future hindbrain	Present		1A
TS13: trunk somite	Present		1A
TS13: trunk unsegmented mesenchyme	Present		1A

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component ectoderm	Present		1B
TS15: 1st branchial arch maxillary component ectoderm	Present		1B
TS15: 2nd branchial arch	Present		1B
TS15: nose	Present	Regionally restricted	1B	Expression is in the olfactory epithelium surrounding the nasal pits.
TS15: forelimb bud ectoderm	Present		1B	Expression is in the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS15: rhombomere 1	Present		1C	Expression extends to the rhombomere 1/2 boundary.
TS15: rhombomere 2	Absent		1C

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present	Regionally restricted	1D	Expression is in the olfactory epithelium surrounding the nasal pits.
TS17: 1st branchial arch mandibular component ectoderm	Present		1D
TS17: 1st branchial arch maxillary component ectoderm	Present		1D
TS17: 2nd branchial arch	Present		1D
TS17: forelimb bud apical ectodermal ridge	Present		1D
TS17: hindlimb bud apical ectodermal ridge	Present		1D

Structure	Level	Pattern	Image	Note
TS17: olfactory epithelium	Present	Regionally restricted	1E	Expression is in the olfactory epithelium surrounding the nasal pits.
TS17: 1st branchial arch mandibular component ectoderm	Present		1E
TS17: 1st branchial arch maxillary component ectoderm	Present		1E
TS17: 2nd branchial arch	Present		1E

Structure	Level	Pattern	Image	Note
TS19: maxilla primordium	Present	Diffuse	1F
TS19: 2nd branchial arch	Present	Diffuse	1F	Expression is in a distal domain.
TS19: mandible primordium	Present	Diffuse	1F	Expression is in a distal domain.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud apical ectodermal ridge	Present		1G
TS17: hindlimb bud mesenchyme	Present	Regionally restricted	1G

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present		1H
TS17: forelimb bud mesenchyme	Present	Regionally restricted	1H	Expression is in the posterior mesenchyme along the entire proximal-distal extent.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present	Regionally restricted	1I	The expression domain is proximally located at the level of the future tarsal/metatarsal elements.

Structure	Level	Pattern	Image	Note
TS19: handplate mesenchyme	Present	Regionally restricted	1J	The expression domain is proximally located at the level of the future tarsal/metatarsal elements and in an anterior domain.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Present	Regionally restricted	3A	Expression is in an anterior and posterior domain.

Structure	Level	Pattern	Image	Note
TS20: forelimb	Weak	Regionally restricted	3B	Expression is reduced in the anterior domain and no consistent change was observed in the posterior domain.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Present	Regionally restricted	3C	Expression is in an anterior and posterior domain.

Structure	Level	Pattern	Image	Note
TS20: hindlimb	Weak	Regionally restricted	3D	Expression is reduced in the anterior domain and no consistent change was observed in the posterior domain.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	3E	Expression is in an anterior and posterior domain.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Weak		3F	Expression is reduced in the anterior domain and no consistent change was observed in the posterior domain.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present		3G	Expression is in an anterior and posterior domain.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Weak		3H	Expression is reduced in the anterior domain and no consistent change was observed in the posterior domain.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		3I

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present		3K

Structure	Level	Pattern	Image	Note
TS19: forelimb bud mesenchyme	Present		3M

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present		3O

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	3Q	Expression is present in a posterior domain.
TS19: footplate mesenchyme	Present		3Q

Structure	Level	Pattern	Image	Note
TS19: footplate	Absent		3R
TS19: hindlimb bud mesenchyme	Present	Regionally restricted	3R	Expression is present in a posterior domain.

Structure	Level	Pattern	Image	Note
TS20: foot mesenchyme	Present		3S
TS20: hindlimb mesenchyme	Present	Regionally restricted	3S	Expression is present in a posterior domain.

Structure	Level	Pattern	Image	Note
TS20: foot	Absent		3T
TS20: hindlimb mesenchyme	Present	Regionally restricted	3T	Expression is present in a posterior domain.

Structure	Level	Pattern	Image	Note
TS22: limb	Absent