Gene Expression Data
Assay Details
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Assay
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Reference:
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J:130339
Minami S, et al., HB-EGF decelerates cell proliferation synergistically with TGFalpha in perinatal distal lung development. Dev Dyn. 2008 Jan;237(1):247-58
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:3785455
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Gene symbol:
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Hbegf
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Gene name:
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heparin-binding EGF-like growth factor
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Newborn lungs were fixed by perfusion of 0.5% glutaraldehyde and equilibrated in a sucrose solution Frozen sections were then fixed with 4% paraformaldehyde. For LacZ staining of adult lungs, procedures of 0.5% glutaraldehyde perfusion and equilibrating in a sucrose solution were omitted.
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Hbegftm1.1Mek/Hbegf+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: lung epithelium |
Present |
Single cells |
2A
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Expression is in scattered epithelial and interstitial cells and is absent in large bronchial epithelial cells.
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| TS27: lung vascular element |
Absent |
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2A
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Absent in vascular endothelial cells.
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Specimen
2B:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Hbegftm1.1Mek/Hbegftm1.1Mek
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: lung epithelium |
Present |
Single cells |
2B
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Expression is in scattered epithelial and interstitial cells and is absent in large bronchial epithelial cells.
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| TS27: lung vascular element |
Absent |
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2B
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Absent in vascular endothelial cells.
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Specimen
2C:
(close )
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Genetic Background:
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involves: C57BL/6 * C57BL/6J * CBA
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Mutant Allele(s):
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Hbegftm1.1Mek/Hbegf+
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: lung epithelium |
Present |
Single cells |
2C
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Expression is in scattered epithelial and interstitial cells and is absent in large bronchial epithelial cells.
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| TS28: lung vascular element |
Absent |
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2C
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Absent in vascular endothelial cells.
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Analysis Tools
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