Probe:	clone 2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted	2C/D	Expression in restricted domains in ventral brain.
TS15: telencephalon	Strong	Regionally restricted	2C/D	Strong expression in ventral telencephalon.
TS15: future midbrain	Present	Regionally restricted	2C/D	Lower expression in ventral midbrain.
TS15: trunk mesenchyme derived from neural crest	Present	Regionally restricted	2C/D	Expression in groups of cells lining the dorsal aorta, presumed to be neural crest-derived precursors of sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	2E/F	Expression in the spinal cord in a group of cells on each side of the floor plate.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	2G/H	Expression extends along length of neural tube to a dorsal and a medial domain.
TS15: trunk mesenchyme derived from neural crest	Present	Regionally restricted	2G/H	Expression in groups of cells lining the dorsal aorta, presumed to be neural crest-derived precursors of sympathetic ganglia.

Structure	Level	Pattern	Image	Note
TS15: future brain	Present	Regionally restricted		No expression in dorsal neuroepithelial of the cerebral cortex, hippocampus, thalamus and cerebellum, or in ventral neuroepithelia of the hypothalamus and lower tegmentum.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted	3A	Authors report that expression domains were the same as at E9.5.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Regionally restricted		No expression in dorsal neuroepithelial of the cerebral cortex, hippocampus, thalamus and cerebellum, or in ventral neuroepithelia of the hypothalamus and lower tegmentum.

Structure	Level	Pattern	Image	Note
TS19: brain ventricular layer	Present	Regionally restricted	3D	Expression first observed in the ventricular zone of the medial part of the lower tegmentum and anterior and posterior hypothalamus.
TS19: midbrain ventricular layer	Present	Regionally restricted	3D	In the upper tegmentum, expression along the whole ventricular zone, only in the portion that is closest to the ventricle; no expression in the deeper part.
TS19: diencephalon lateral wall ventricular layer	Present	Regionally restricted	3D	Expression in ventricular cells throughout hypothalamus, except for a small triangle-shaped region covering full depth of ventricular zone at level of mammillary recess - may correspond the rudiment of the mammillary complex.
TS19: cerebellum primordium	Absent		3D

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present	Regionally restricted		Expression in the ventricular zone throughout the CNS, although at different levels in different regions. In many regions, expression was not seen in all cells of the ventricular zone.
TS20: spinal cord ventricular layer	Present	Regionally restricted		Expression in the ventricular zone throughout the CNS, although at different levels in different regions. In many regions, expression was not seen in all cells of the ventricular zone.
TS20: cerebral cortex ventricular layer	Present			Authors state that expression in the ventricular zone of the cerebral cortex started at E11.5-12.5, although it did not reach the high levels observed elsewhere in the CNS at the same stages.
TS20: autonomic nervous system	Present	Regionally restricted		Expression in neural crest-derived cells: in enteric ganglia surrounding gut, sympatho-adrenal lineage in adrenal medulla, sympathetic and coeliacum ganglia, parasympathetic lineage in sphenopalatine ganglion, and in ganglia along lung and tongue.
TS20: sympathetic ganglion	Present			Expression in neural crest-derived cells: in enteric ganglia surrounding gut, sympatho-adrenal lineage in adrenal medulla, sympathetic and coeliacum ganglia, parasympathetic lineage in sphenopalatine ganglion, and in ganglia along lung and tongue.

Structure	Level	Pattern	Image	Note
TS20: cerebellum primordium	Present	Regionally restricted	3G	Expression in the ventricular zone of the hippocampus and cerebellum.
TS20: telencephalon ventricular layer	Present	Regionally restricted	3G	Expression in the ventricular zone of the hippocampus and cerebellum.
TS20: thalamus ventricular layer	Present		3G
TS20: hypothalamus ventricular layer	Present	Regionally restricted	3G	No expression in a small triangle-shaped region of the hypothalamic ventricular zone that may correspond the rudiment of the mammillary complex.
TS20: hindbrain	Present	Spotted	3G	Expression in scattered cells in the spinal cord, hindbrain and midbrain.
TS20: midbrain	Present	Spotted	3G	Expression in scattered cells in the spinal cord, hindbrain and midbrain.
TS20: spinal cord	Present	Spotted	3G	Expression in scattered cells in the spinal cord, hindbrain and midbrain.

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Strong		3J	Expression in the ventricular zone of all regions of the brain.

Structure	Level	Pattern	Image	Note
TS21: hindbrain	Present	Spotted	4A/B	Expression in scattered cells in the spinal cord, hindbrain and midbrain.
TS21: midbrain	Present	Spotted	4A/B	Expression in scattered cells in the spinal cord, hindbrain and midbrain.
TS21: spinal cord	Present	Spotted	4A/B	Expression in scattered cells in the spinal cord, hindbrain and midbrain.

Structure	Level	Pattern	Image	Note
TS21: corpus striatum	Present	Regionally restricted		Expression in clusters of cells in the striatal and mediodorsal thalamic area.
TS21: thalamus	Present	Regionally restricted		Expression in clusters of cells in the striatal and mediodorsal thalamic area.

Structure	Level	Pattern	Image	Note
TS28: cerebellum	Present	Regionally restricted	4C/D	Expression in clusters of cells in the cerebellum.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present	Regionally restricted	4E/F	Expression in clusters of cells in the hippocampal area.

Structure	Level	Pattern	Image	Note
TS28: hindbrain	Present	Spotted		Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).
TS28: midbrain	Present	Spotted		Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).
TS28: spinal cord	Present	Spotted		Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).
TS28: brainstem	Present	Regionally restricted		Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).
TS28: inferior colliculus	Present			Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).
TS28: superior colliculus	Present			Expression in scattered cells in the spinal cord, hindbrain and midbrain, particularly in the ventral hindbrain (reticular formation) and dorsal midbrain (interior and superior colliculus).

Structure	Level	Pattern	Image	Note
TS28: brain	Trace	Regionally restricted	4G/H	No expression except for a few cells around the lateral ventricles; probably correspond to subependymal cells.

Structure	Level	Pattern	Image	Note
TS15: olfactory placode	Strong		5A/B	High level of expression in the nasal placodes.

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present	Regionally restricted	5C/D	Widespread, but not uniform, expression in the olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS25: olfactory epithelium	Present	Regionally restricted	5E/F	High levels of expression in cells in basal positions, presumeably corresponding to neuronal precursors. No expression in sustentacular cells, and their dividing precursors, and in post-mitotic olfactory neurons.

Structure	Level	Pattern	Image	Note
TS15: optic vesicle	Weak	Regionally restricted		Low level of expression in limitied portions of the optic vesicles.

Structure	Level	Pattern	Image	Note
TS17: eye	Weak	Regionally restricted	6A/B	Low level of expression in limitied portions of the optic vesicles.

Structure	Level	Pattern	Image	Note
TS19: retina	Absent

Structure	Level	Pattern	Image	Note
TS20: retina	Absent		6C

Structure	Level	Pattern	Image	Note
TS21: retina	Absent

Structure	Level	Pattern	Image	Note
TS22: retina	Weak	Regionally restricted		Low level of expression in the retina, throughout the ventricular or neuroblast layer in the peripheral as well as central retina, but not in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: retina	Strong	Regionally restricted	6F	Higher level of expression in the retina, but not in the ganglion cell layer.`
TS23: neural retina	Present	Regionally restricted	6F	Expression throughout the ventricular or neuroblast layer in the peripheral retina, as well as in the central retina.

Structure	Level	Pattern	Image	Note
TS27: retina	Present	Regionally restricted	6I/J	Expression in clusters of cells in the retina.
TS27: neural retina	Weak	Regionally restricted	6I/J	Low level of expression in the neuroblast layer.
TS27: retina inner nuclear layer	Present		6I/J