Sftpb Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:1959 D'Amore-Bruno MA, et al., Sequence, ontogeny, and cellular localization of murine surfactant protein B mRNA. Am J Physiol. 1992 Jan;262(1 Pt 1):L40-7
Assay type:	Northern blot
MGI Accession ID:	MGI:3774940
Gene symbol:	Sftpb
Gene name:	surfactant associated protein B

Probe:	SP-B probe4
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography

Results

Image: 6

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	1.6 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
DAY 15	E15.0 (a)	TS23: lung	Weak (b)	20 µg; total RNA	Not Specified		Not Specified
DAY 16	E16.0 (a)	TS24: lung	Weak	20 µg; total RNA	Not Specified		Not Specified
DAY 17	E17.0 (a)	TS25: lung	Present (c)	20 µg; total RNA	Not Specified		Not Specified
DAY 18	E18.0 (a)	TS26: lung	Strong (d)	20 µg; total RNA	Not Specified		Not Specified
A	P adult	TS28: lung	Strong	20 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression detected at low levels at day 15.
(c) Marked increase in expression between days 16 and 17.
(d) Expression at adult levels by day 18 of gestation.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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