Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Snord116tm1.1Uta
Name:	small nucleolar RNA, C/D box 116 cluster; targeted mutation 1.1, Uta Francke
MGI ID:	MGI:3773673
Synonyms:	1-lox, 1-loxp, Snord116del
Gene:	Snord116  Location: unknown  Genetic Position: Chr7, Syntenic

Germline Transmission:	Earliest citation of germline transmission: J:131427
Parent Cell Line:	Bruce 4 (ES Cell)
Strain of Origin:	B6.Cg-Thy1a

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Snord116tm1.1Uta involves 9 genes/genome features (Snord116, Snord116l2, Snord116l8 ...) View all   Mutation details: Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived Bruce-4 embryonic stem (ES) cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with a cre expressing plasmid. The resulting 1-loxP ES cells (with the Snord116 cluster deleted and a single loxP site remaining) were injected into recipient blastocysts. (J:131427)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Snord116 Mutation:	3 strains or lines available

Original:	J:131427 Ding F, et al., SnoRNA Snord116 (Pwcr1/MBII-85) Deletion Causes Growth Deficiency and Hyperphagia in Mice. PLoS One. 2008;3(3):e1709
All:	13 reference(s)