Probe:	MH1041 probe 904
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3A Aldoa
TS22: midbrain	Weak		3A Aldoa
TS22: cerebellum	Weak		3A Aldoa
TS22: pons	Moderate		3A Aldoa
TS22: medulla oblongata	Strong		3A Aldoa
TS22: spinal cord	Moderate		3A Aldoa
TS22: cranial ganglion	Strong		3A Aldoa
TS22: dorsal root ganglion	Strong		3A Aldoa
TS22: olfactory epithelium	Strong		3A Aldoa
TS22: intestine	Strong		3A Aldoa
TS22: salivary gland	Strong		3A Aldoa
TS22: tongue	Strong		3A Aldoa
TS22: stomach	Strong		3A Aldoa
TS22: liver	Weak		3A Aldoa
TS22: lung	Moderate		3A Aldoa
TS22: metanephros	Weak		3A Aldoa
TS22: thyroid gland	Weak		3A Aldoa
TS22: pituitary gland	Strong		3A Aldoa
TS22: pancreas	Weak		3A Aldoa
TS22: adrenal gland	Weak		3A Aldoa
TS22: heart	Strong		3A Aldoa
TS22: skeletal muscle	Strong		3A Aldoa
TS22: cartilage	Absent		3A Aldoa	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Aldoa	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: musculature	Strong		MH1041_4A	There is strong expression in all muscles.
TS22: appendicular skeleton	Strong		MH1041_4A
TS22: axial skeleton	Strong		MH1041_4A
TS22: cardiovascular system	Strong		MH1041_4A
TS22: metanephros	Strong		MH1041_4A
TS22: liver	Present	Single cells	MH1041_4A	Expression is present in numerous scattered cells.
TS22: spinal cord mantle layer	Present	Regionally restricted	MH1041_4A	Expression is present in the motor column.
TS22: brain	Present		MH1041_4A
TS22: cranial nerve	Present		MH1041_4A	There is elevated expression in this structure.
TS22: piriform cortex	Present		MH1041_4A	There is elevated expression in this structure.
TS22: pons	Present		MH1041_4A	There is elevated expression in this structure.