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Abcg2tm2Bsor
Targeted Allele Detail
Summary
Symbol: Abcg2tm2Bsor
Name: ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 2, Brian P Sorrentino
MGI ID: MGI:3768641
Synonyms: Abcg2GFP
Gene: Abcg2  Location: Chr6:58561476-58669436 bp, + strand  
Alliance: Abcg2tm2Bsor page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:128682
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129S4/SvJae
Mutation
description
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
 
Mutation detailsA loxP-flanked neomycin selection cassette and an IRES-GFP reporter gene were inserted immediately downstream of the stop codon and upstream of the endogenous polyadenylation signal sequence. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. Reporter gene expression was confirmed by immunohistochemistry on intestine and kidney from homozygotes. (J:128682)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Abcg2 Mutation:  74 strains or lines available
References
Original:  J:128682 Tadjali M, et al., Prospective isolation of murine hematopoietic stem cells by expression of an Abcg2/GFP allele. Stem Cells. 2006 Jun;24(6):1556-63
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory