Abcg2tm2Bsor
Targeted Allele Detail
|
Symbol: |
Abcg2tm2Bsor |
Name: |
ATP binding cassette subfamily G member 2 (Junior blood group); targeted mutation 2, Brian P Sorrentino |
MGI ID: |
MGI:3768641 |
Synonyms: |
Abcg2GFP |
Gene: |
Abcg2 Location: Chr6:58561476-58669436 bp, + strand
|
Alliance: |
Abcg2tm2Bsor page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:128682
|
Parent Cell Line: |
Not Specified (ES Cell)
|
Strain of Origin: |
129S4/SvJae
|
|
Allele Type: |
|
Targeted (Reporter) |
Mutation: |
|
Insertion
|
|
|
Mutation details: A loxP-flanked neomycin selection cassette and an IRES-GFP reporter gene were inserted immediately downstream of the stop codon and upstream of the endogenous polyadenylation signal sequence. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. Cre-mediated excision subsequently removed the neomycin cassette upon transmission from male chimeric mice. Reporter gene expression was confirmed by immunohistochemistry on intestine and kidney from homozygotes.
(J:128682)
|
|
|
|
Original: |
J:128682 Tadjali M, et al., Prospective isolation of murine hematopoietic stem cells by expression of an Abcg2/GFP allele. Stem Cells. 2006 Jun;24(6):1556-63 |
All: |
3 reference(s) |
|