Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:128043
Modamio-Hoybjor S, et al., A mutation in CCDC50, a gene encoding an effector of epidermal growth factor-mediated cell signaling, causes progressive hearing loss. Am J Hum Genet. 2007 Jun;80(6):1076-89
|
|
Assay type:
|
RT-PCR
|
|
MGI Accession ID:
|
MGI:3766785
|
|
Gene symbol:
|
Ccdc50
|
|
Gene name:
|
coiled-coil domain containing 50
|
|
Probe:
|
F7-R1 primer pair
|
|
Assay notes:
|
The samples were amplified with the F1-R4 primer pair (MGI:3766633) as shown in assay: MGI:3766656 and then with this nested primer pair. In addiiton, these samples were digested with BseLI. Restriction pattern of transcript 1 (with exon 8) includes three bands of 522 bp, 445 bp, and 76 bp. Transcript 2 (without exon 8) digestion yields two bands of 922 bp and 76 bp.
|
|
|
Results
|
Image: 3B
| Mol. Wt. Marker Lane |
| Control |
| Control |
| P58
|
TS28: lung
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
| P58
|
TS28: brain
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
| P58
|
TS28: liver
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
| E18.5
|
TS26: cochlea
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
| P0
|
TS27: cochlea
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
| P14
|
TS28: cochlea
|
Present
|
Present
|
Present
|
Present
|
1 µg; total RNA |
Not Specified |
| Not Specified |
|
Analysis Tools
