Antibody:	TES101 mAb
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Fixative was 4% paraformaldehyde and 0.1% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS20: testis	Absent

Structure	Cell Type	Level	Pattern	Image	Note
TS22: germ cell of testis		Present	Regionally restricted	1A	Expression is on the cell surfaces of some prospermatogonia and is absent in Sertoli and interstitial cells.
TS22: interstitium of the testis		Absent		1A	Expression is on the cell surfaces of some prospermatogonia and is absent in Sertoli and interstitial cells.
TS22: Sertoli cell	Sertoli cell	Absent		1A	Expression is on the cell surfaces of some prospermatogonia and is absent in Sertoli and interstitial cells.

Structure	Level	Pattern	Image	Note
TS24: seminiferous cord	Present	Regionally restricted	1C	Expression is on the cell surfaces of most prospermatogonia.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: germ cell of testis		Present		1E	Positive cells are toward the basement membrane of the seminiferous tubule, and formed an arrangement around the basement membrane.
TS27: Sertoli cell	Sertoli cell	Absent		1E	Absent in Sertoli cells and other cell types.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present		1F	Positive cells are toward the basement membrane of the seminiferous tubule, and formed an arrangement around the basement membrane.
TS28: Sertoli cell	Sertoli cell	Absent		1F	Absent in Sertoli cells and other cell types.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present		1G	Positive cells are toward the basement membrane of the seminiferous tubule, and formed an arrangement around the basement membrane.
TS28: Sertoli cell	Sertoli cell	Absent		1G	Absent in Sertoli cells and other cell types.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present		1H	Positive cells are toward the basement membrane of the seminiferous tubule, and formed an arrangement around the basement membrane.
TS28: Sertoli cell	Sertoli cell	Absent		1H	Absent in Sertoli cells and other cell types.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Present	Single cells	1I	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatocyte		Present	Single cells	1I	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatogonium		Absent		1I
TS28: Sertoli cell	Sertoli cell	Absent		1I
TS28: interstitium of the testis		Absent		1I
TS28: Leydig cell	Leydig cell	Absent		1I

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Present	Single cells	1J	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatocyte		Present	Single cells	1J	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatogonium		Absent		1J
TS28: Sertoli cell	Sertoli cell	Absent		1J	Absent in Sertoli cells, interstitial cells, including Leydig cells.
TS28: interstitium of the testis		Absent		1J
TS28: Leydig cell	Leydig cell	Absent		1J

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Present	Single cells	1K	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatocyte		Present	Single cells	1K	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatogonium		Absent		1K
TS28: Sertoli cell	Sertoli cell	Absent		1K
TS28: interstitium of the testis		Absent		1K
TS28: Leydig cell	Leydig cell	Absent		1K

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Present	Single cells	1M	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatocyte		Present	Single cells	1M	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatogonium		Absent		1M
TS28: Sertoli cell	Sertoli cell	Absent		1M
TS28: interstitium of the testis		Absent		1M
TS28: Leydig cell	Leydig cell	Absent		1M

Structure	Cell Type	Level	Pattern	Image	Note
TS28: spermatid		Present	Single cells	1N	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatocyte		Present	Single cells	1N	Expression is in the surface of spermatocytes and spermatids.
TS28: spermatogonium		Absent		1N
TS28: Sertoli cell	Sertoli cell	Absent		1N
TS28: interstitium of the testis		Absent		1N
TS28: Leydig cell	Leydig cell	Absent		1N

Structure	Level	Pattern	Image	Note
TS20: ovary	Absent

Structure	Level	Pattern	Image	Note
TS22: germ cell of ovary	Present	Regionally restricted	2A	Expression is on the cell surfaces of the germ cells.

Structure	Level	Pattern	Image	Note
TS24: germ cell of ovary	Present		2B

Structure	Level	Pattern	Image	Note
TS26: germ cell of ovary	Present		2C

Structure	Level	Pattern	Image	Note
TS27: germ cell of ovary	Present	Regionally restricted	2D	Expression is on the surface of germ cells clustered in the cortex.

Structure	Level	Pattern	Image	Note
TS28: germ cell of ovary	Absent		2E
TS28: ovary follicle	Absent		2E

Structure	Level	Pattern	Image	Note
TS28: germ cell of ovary	Absent		2F
TS28: ovary follicle	Absent		2F