Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | Notes | References

Symbol:	Fn1tm4Hyn
Name:	fibronectin 1; targeted mutation 4, Richard Hynes
MGI ID:	MGI:3764511
Synonyms:	EIIIA-EIIIB-, EIIIA/EIIIB-double-null
Gene:	Fn1  Location: Chr1:71624679-71692359 bp, - strand  Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
Alliance:	Fn1tm4Hyn page

Germline Transmission:	Earliest citation of germline transmission: J:126737
Parent Cell Line:	Other (see notes) (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutation:    Intragenic deletion   Mutation details: ES cells from homozygous Fn1tm1Ksek male mouse in which exon EDB (EIIIB) was deleted and replaced with a singe loxp site was isolated and targeted with the same construct used in Fn1tm1Bwg. Following homologous recombination and transient cre-mediated recombination, a single loxp site was left in place of the exon encoding the EIIIA domain. Northern and Western blot confirmed the absence of EIII1 and EIIIB mRNA and protein and that deletion of both of those exons did not compromise the expression of FN mRNA and protein, nor the deposition of FN into the matrix. (J:126737)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

9 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Fn1 Mutation:	129 strains or lines available

Original:	J:126737 Astrof S, et al., Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin. Dev Biol. 2007 Nov 1;311(1):11-24
All:	3 reference(s)