Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(CAG-Ub*G76V/GFP)1Dant
Name:	transgene insertion 1, Nico Dantuma
MGI ID:	MGI:3762797
Synonyms:	Tg(ACTB-Ub*G76V/GFP)1Dant, Tg(CAG-Ub*G76V/GFP)1Dant, UbG76V-GFP, UbG76V-GFP/1, UbG76V-GFP/2, UPS reporter
Transgene:	Tg(CAG-Ub*G76V/GFP)1Dant  Location: unknown
Alliance:	Tg(CAG-Ub*G76V/GFP)1Dant page

Strain of Origin:

(CBA x C57BL/6)F1

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: The UbG76V-GFP transgene was designed with a chicken beta-actin promoter (and cytomegalovirus (CMV) immediate early enhancer) upstream of a green fluorescent protein (GFP) fused to a mutant ubiquitin moiety (UbG76V). Although transcripts of the UbG76V-GFP fusion gene are detected in all tissues examined, the G76V substitution prevents removal of the ubiquitin moiety leading to efficient ubiquitination and proteasomal degradation of the fusion protein (and thus no fluorescence) in these tissues. Line 1 with high expression was maintained by backcrosses to C57BL/6N mice. The high expressing line (UbG76V-GFP/1) has very low, but detectable, fluorescence expression in brain, spinal cord, and exocrine pancreatic tissue prior to administration of proteasome inhibitors. (J:127085, J:127087)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:127085 Lindsten K, et al., A transgenic mouse model of the ubiquitin/proteasome system. Nat Biotechnol. 2003 Aug;21(8):897-902
All:	15 reference(s)