Tg(Thy1-Brainbow2.1)RLich
Transgene Detail
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Symbol: |
Tg(Thy1-Brainbow2.1)RLich |
Name: |
transgene insertion R, Jeff W Lichtman |
MGI ID: |
MGI:3761284 |
Synonyms: |
Tg(Thy1-hrGFPII,-mYFP,-tdimer2(12),-mCerulean)R21Lich, Thy1-Brainbow-2.1 line R |
Transgene: |
Tg(Thy1-Brainbow2.1)RLich Location: unknown
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Alliance: |
Tg(Thy1-Brainbow2.1)RLich page
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Strain of Origin: |
C57BL/6J x CBA
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Transgene Type: |
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Transgenic (Reporter) |
Mutation: |
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Insertion
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Mutation details: The Thy1-Brainbow 2.1 transgene was designed with the mouse Thy1 regulatory elements surrounding four fluorescent proteins (green (GFP), enhanced yellow (EYFP), red (RFP), and cyan (CFP)) sequences uniquely positioned in a tandem fashion and delimited by LoxP sites in opposite orientation. Specifically, this Brainbow 2.1 coding region is composed of two adjacent head to tail tandem dimers, the first contains a LoxP site, humanized Renilla GFP (hrGFPII (Stratagene); with nuclear localization signal), and polyA sequence in forward orientation, and a LoxP site, monomeric EYFP (mYFPA206K), and polyA sequence in reverse orientation. The second head to tail dimer contains a LoxP site, tdimer2(12) RFP, and polyA sequence in forward orientation, and a LoxP site, mCerulean CFP (with membrane tethering palmitoylation sequence), and polyA sequence in reverse orientation. A single FRT site is located at the 3' end of the coding region.
The hrGFPII variant of GFP (from Stratagene vector phrGFPII-C) has amino acid substitutions designed to improve spectral properties and performance in mammalian systems. The monomeric EYFP (mYFPA206K) has an amino acid substitution replacing a hydrophobic region with a positively charged residue designed to prevent dimerization. The tdimer2(12) RFP is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer). The monomeric Cerulean (mCerulean) is a variant of ECFP (ECFPS72A/Y145A/H148D/A206K) with amino acid substitutions designed to improve spectral properties and prevent dimerization.
(J:125961)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:125961 Livet J, et al., Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature. 2007 Nov 1;450(7166):56-62 |
All: |
2 reference(s) |
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