Gch1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122522 Gil M, et al., Regulation of GTP cyclohydrolase I expression by orphan receptor Nurr1 in cell culture and in vivo. J Neurochem. 2007 Apr;101(1):142-50
Assay type:	RT-PCR
MGI Accession ID:	MGI:3760519
Gene symbol:	Gch1
Gene name:	GTP cyclohydrolase 1

Probe:	GTPCH-pA, GTPCH-pB
Probe preparation:	labelled with fluorescein
Assay notes:	Quantitative real-time PCR assay.

Results

Image: 1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
WT	E12.5	TS20: midbrain	Present	Not Specified; total RNA	Swiss Webster		Not Specified	Ventral midbrain.
KO	E12.5	TS20: midbrain	Present (a)	Not Specified; total RNA	involves: 129S4/SvJae	Nr4a2^tm1Niko/Nr4a2^tm1Niko	Not Specified	Ventral midbrain.
WT	P0	TS27: midbrain	Present (b)	Not Specified; total RNA	Swiss Webster		Not Specified	Ventral midbrain.
KO	P0	TS27: midbrain	Present (c)	Not Specified; total RNA	involves: 129S4/SvJae	Nr4a2^tm1Niko/Nr4a2^tm1Niko	Not Specified	Ventral midbrain.

Notes:
(a) The mRNA levels were decreased about 70% compared wild-type.
(b) Expression increases more than twofold between E12.5 and birth.
(c) Expression levels are the same as found at E12.5 in this mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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