Gba1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74199 Ponce E, et al., Temporal and Spatial Expression of Murine Acid beta-Glucosidase mRNA. Mol Genet Metab. 2001 Dec;74(4):426-34
Assay type:	Northern blot
MGI Accession ID:	MGI:3706693
Gene symbol:	Gba1
Gene name:	glucosylceramidase beta 1

Probe:	GCase cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	The results were not shown.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.5 kb (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
Lane 1	P adult	TS28: liver	Strong (b)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 2	P adult	TS28: metanephros	Present (c)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 3	P adult	TS28: heart	Present (d)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 4	P adult	TS28: lung	Weak (e)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 5	P adult	TS28: brain	Weak (f)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 6	P adult	TS28: testis	Weak (g)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Male
Lane 7	P adult	TS28: spleen	Weak (h)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 8	P adult	TS28: skeletal muscle	Weak (i)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified

Notes:
(a) Minor bands of 5.2 and 2.1kb were also detected but were not discussed.
(b) The highest level of adult expression was detected in liver (assigned 100%).
(c) Expression level was 36% in kidney.
(d) Expression level was 21% in heart.
(e) Expression level was 14% in lung.
(f) Expression level was 13% in brain.
(g) Expression level was 12% in testis.
(h) Expression level was 7% in spleen.
(i) Expression level was 7% in skeletal muscle.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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