Gba1 Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:74199 Ponce E, et al., Temporal and Spatial Expression of Murine Acid beta-Glucosidase mRNA. Mol Genet Metab. 2001 Dec;74(4):426-34
Assay type:	Northern blot
MGI Accession ID:	MGI:3706688
Gene symbol:	Gba1
Gene name:	glucosylceramidase beta 1

Probe:	GCase cDNA1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Phosphorimaging
Assay notes:	The results were not shown.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.5 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
Lane 1	E7.0 (a)	TS10: embryo	Strong (c)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 2	E11.0 (a)	TS18: embryo	Weak (d)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 3	E15.0 (a)	TS23: embryo	Present (e)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified
Lane 4	E17.0 (a)	TS25: embryo	Present (f)	2 µg; poly-A+ RNA	involves: BALB/c * Swiss Webster		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Minor bands of 5.2 and 2.1kb were also detected but were not discussed.
(c) The highest level of prenatal expression was detected at E7 (assigned 100%).
(d) The lowest level of prenatal expression was detected at E11 (13%).
(e) Expression level was 40% at E15.
(f) Expression level was 30% at E17.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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