Tor1b Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:106947 Vasudevan A, et al., Developmental patterns of torsinA and torsinB expression. Brain Res. 2006 Feb 16;1073-1074C:139-145
Assay type:	Northern blot
MGI Accession ID:	MGI:3702748
Gene symbol:	Tor1b
Gene name:	torsin family 1, member B

Probe:	TorsinB probe1
Probe preparation:	Double stranded labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	Signal intensity relative to control was presented in a graph in Fig. 1C. Additional less abundant messages were noted on the blot.

Results

Image: 1B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	3.4 kb (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E11	E11.0 (a)	TS19: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
E13	E13.0 (a)	TS21: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
E17	E17.0 (a)	TS25: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
P0	P0	TS27: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
P7	P7	TS28: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
P14	P14	TS28: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
P21	P21	TS28: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified
P30	P30	TS28: brain	Present	2 µg; poly-A+ RNA	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression level increased during embryonic and postnatal stages with the highest level at P14. The level rose 2.9 fold between E11 and P14, then were maintained until P30.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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