Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gsk3btm1Grc
Name:	glycogen synthase kinase 3 beta; targeted mutation 1, Gerald R Crabtree
MGI ID:	MGI:3701999
Synonyms:	GSK-3betaFRB*
Gene:	Gsk3b  Location: Chr16:37909363-38066446 bp, + strand  Genetic Position: Chr16, 26.54 cM, cytoband B4
Alliance:	Gsk3btm1Grc page

Germline Transmission:	Earliest citation of germline transmission: J:100153
Parent Cell Line:	TC1/TC-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A mutant FRB tag, composed of an 89 amino acids with three point mutations (K2095P, T2098L, and W2101F), was targeted into the C terminus of the locus. A floxed neo cassette was inserted into the last intron and an FRB*HA sequence placed immediately before the stop codon. The neo was subsequently removed via crossing with mice expressing cre recombinase in the germline. Presence of this mutant FRB tag destabilizes the protein resulting in decrease or loss of expression. Addition of rapamycin or a rapamycin analogue stabilizes the protein and restores expression. (J:100153)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gsk3b Mutation:	111 strains or lines available

Original:	J:100153 Stankunas K, et al., Conditional protein alleles using knockin mice and a chemical inducer of dimerization. Mol Cell. 2003 Dec;12(6):1615-24
All:	5 reference(s)