Grpr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:87830 Shan L, et al., Bombesin-like peptide receptor gene expression, regulation, and function in fetal murine lung. Am J Physiol Lung Cell Mol Physiol. 2004 Jan;286(1):L165-73
Assay type:	RT-PCR
MGI Accession ID:	MGI:3701444
Gene symbol:	Grpr
Gene name:	gastrin releasing peptide receptor

Probe:	Grpr-pE, Grpr-pF
Visualized with:	Autoradiography
Assay notes:	Each lane was a pooled sample from 2-5 litters. The RT-PCR reaction was blotted and hybridized with an internal oligonucleotide probe. The autoradiogram was pictured in Fig. 1A and a densitometric analysis was presented in a graph in Fig. 1B.

Results

Image: 1A GRP-R

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	334 bp (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E12	E12.0 (a)	TS20: lung	Present	Not Specified; total RNA	Swiss Webster		Not Specified
E13	E13.0 (a)	TS21: lung	Present (c)	Not Specified; total RNA	Swiss Webster		Not Specified
E14	E14.0 (a)	TS22: lung	Trace (c)	Not Specified; total RNA	Swiss Webster		Not Specified
E15	E15.0 (a)	TS23: lung	Present (c)	Not Specified; total RNA	Swiss Webster		Not Specified
E16	E16.0 (a)	TS24: lung	Present (d)	Not Specified; total RNA	Swiss Webster		Not Specified
E17	E17.0 (a)	TS25: lung	Present	Not Specified; total RNA	Swiss Webster		Not Specified
E18	E18.0 (a)	TS26: lung	Present	Not Specified; total RNA	Swiss Webster		Not Specified
P1	P1	TS27: lung	Weak	Not Specified; total RNA	Swiss Webster		Not Specified
P2	P2	TS27: lung	Weak	Not Specified; total RNA	Swiss Webster		Not Specified
P4	P4	TS28: lung	Weak	Not Specified; total RNA	Swiss Webster		Not Specified
P7	P7	TS28: lung	Weak	Not Specified; total RNA	Swiss Webster		Not Specified
P14	P14	TS28: lung	Weak	Not Specified; total RNA	Swiss Webster		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was detected at E12, peaked at E16 and then fell off to very low levels after birth.
(c) The strength of this band was assigned by the curator based on the graph depicted in Fig. 1B.
(d) Expression peaked at E16.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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