Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Igkj1tm1(IgkHyHEL10)Mnz
Name:	immunoglobulin kappa joining 1; targeted mutation 1, Michel C Nussenzweig
MGI ID:	MGI:3694788
Synonyms:	Tg(Igk-V22-33-aHEL)1Mnz, VkalphaHEL
Gene:	Igkj1  Location: Chr6:70699546-70699583 bp, + strand  Genetic Position: Chr6, 32.04 cM
Alliance:	Igkj1tm1(IgkHyHEL10)Mnz page

Germline Transmission:	Earliest citation of germline transmission: J:115079
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutation:    Insertion   Mutation details: The recombined immunoglobulin genes present in the HyHEL10 hybridoma produce antibodies with high affinity for hen egg lysozyme (HEL). The recombined murine Igk variable and joining gene segments present in this hybridoma were placed under the control of a Vkappa22-33 promoter. A floxed neomycin resistance cassette was placed upstream and the entire construct replaced the endogenous Igk-J1 gene segment. Mice were crossed with cre-transgenic mice to removed the floxed neomycin cassette. The transgenic IgK light chain is detectable on some B cells in heterozygote mice. (J:115079)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Igkj1 Mutation:	2 strains or lines available

Original:	J:115079 Casellas R, et al., Contribution of receptor editing to the antibody repertoire. Science. 2001 Feb 23;291(5508):1541-4
All:	3 reference(s)