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Gene Expression Data
Assay Details
Assay
Reference: J:112887 Mori AD, et al., Tbx5-dependent rheostatic control of cardiac gene expression and morphogenesis. Dev Biol. 2006 Sep 15;297(2):566-86
Assay type: RT-PCR
MGI Accession ID: MGI:3692962
Gene symbol: Tbx5
Gene name: T-box 5
Probe: Tbx5Ex3-TM-FOR, Tbx5Ex3-TM-REV
Assay notes: This assay was a quantitative real-time PCR. The probe sequence is listed in MGI:3692587. The results were presented graphically.
Results
Image: 1C ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 E8.5 (a) TS13: embryo Present Not Specified; total RNA Black Swiss Not Specified
Lane 2 E8.5 (a) TS13: embryo Present Not Specified; total RNA 129S/SvEv Not Specified
Lane 3 E8.5 (a) TS13: embryo Present (b) Not Specified; total RNA involves: 129S/SvEv Tbx5tm1Jse/Tbx5+Not Specified
Lane 4 E8.5 (a) TS13: embryo Present (c) Not Specified; total RNA either: (involves: 129/Sv) or (involves: Black Swiss) Tbx5tm1.1Jse/Tbx5+Not Specified
Lane 5 E8.5 (a) TS13: embryo Present (d) Not Specified; total RNA involves: 129S/SvEv Tbx5tm1Jse/Tbx5tm1JseNot Specified
Lane 6 E8.5 (a) TS13: embryo Absent Not Specified; total RNA either: (involves: 129/SvEv) or (involves: Black Swiss) Tbx5tm1.1Jse/Tbx5tm1.1JseNot Specified
Lane 7 E12.5 (a) TS20: heart Present Not Specified; total RNA Black Swiss Not Specified
Lane 8 E12.5 (a) TS20: heart Present Not Specified; total RNA 129S/SvEv Not Specified
Lane 9 E12.5 (a) TS20: heart Present (e) Not Specified; total RNA involves: 129S/SvEv Tbx5tm1Jse/Tbx5+Not Specified
Lane 10 E12.5 (a) TS20: heart Present (f) Not Specified; total RNA either: (involves: 129/Sv) or (involves: Black Swiss) Tbx5tm1.1Jse/Tbx5+Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Average amount of functional transcript is 78% of wild type.
(c) Average amount of functional transcript is 30% of wild type.
(d) Average amount of functional transcript is 22% of wild type.
(e) Average amount of functional transcript is 67% of wild type.
(f) Average amount of functional transcript is 55% of wild type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory