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Tg(tetO-EGFP,-Tgfbr2)8Mcle
Transgene Detail
Summary
Symbol: Tg(tetO-EGFP,-Tgfbr2)8Mcle
Name: transgene insertion 8, Ian S McLennan
MGI ID: MGI:3691946
Synonyms: PTR, PTRh, PTR (line 8)
Transgene: Tg(tetO-EGFP,-Tgfbr2)8Mcle  Location: unknown  
Alliance: Tg(tetO-EGFP,-Tgfbr2)8Mcle page
Transgene
origin
Strain of Origin:  FVB/N
Transgene
description
Transgene Type:    Transgenic (Dominant negative, Inducible, Inserted expressed sequence, Reporter)
Inducer:    doxycycline/tetracycline
Mutation:    Insertion
 
Mutation detailsThe transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant-negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline. (J:109835)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Notes
Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis.
References
Original:  J:109835 Frugier T, et al., Transgenic mice carrying a tetracycline-inducible, truncated transforming growth factor beta receptor (TbetaRII). Genesis. 2005 May;42(1):1-5
All:  6 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory