Tg(tetO-EGFP,-Tgfbr2)8Mcle
Transgene Detail
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Symbol: |
Tg(tetO-EGFP,-Tgfbr2)8Mcle |
Name: |
transgene insertion 8, Ian S McLennan |
MGI ID: |
MGI:3691946 |
Synonyms: |
PTR, PTRh, PTR (line 8) |
Transgene: |
Tg(tetO-EGFP,-Tgfbr2)8Mcle Location: unknown
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Alliance: |
Tg(tetO-EGFP,-Tgfbr2)8Mcle page
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Transgene Type: |
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Transgenic (Dominant negative, Inducible, Inserted expressed sequence, Reporter) |
Inducer: |
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doxycycline/tetracycline |
Mutation: |
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Insertion
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Mutation details: The transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant-negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline.
(J:109835)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis.
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Original: |
J:109835 Frugier T, et al., Transgenic mice carrying a tetracycline-inducible, truncated transforming growth factor beta receptor (TbetaRII). Genesis. 2005 May;42(1):1-5 |
All: |
6 reference(s) |
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