Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Apobtm1Mae
Name:	apolipoprotein B; targeted mutation 1, Nobuyo Maeda
MGI ID:	MGI:3665449
Synonyms:	apo b81
Gene:	Apob  Location: Chr12:8027648-8066835 bp, + strand  Genetic Position: Chr12, 3.53 cM
Alliance:	Apobtm1Mae page

Germline Transmission:	Earliest citation of germline transmission: J:36426
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted Mutation:    Insertion   Mutation details: Using an 'in step' recombination strategy, the sequence encoding the putative binding domains in exon 26 were replaced with those encoding human HBB peptides. The 5' binding domain (3143LSVKAQYKKNSD3154) was replaced with amino acids 1-12 of the HBBS globin (1VHLTPVEKSAVT12). The 3' binding domain (3356GTSRLMRKRGL3366) was replaced with amino acids 120-131 of HBBA globin (120KEFTPPVQAAYQ131). The reading frame was maintained in the mutant allele, however, the disruption creates a premature stop codon at position 3688. The mutant allele contains 3687 residues, which is 81% of the full length protein. (J:36426)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Apob Mutation:	221 strains or lines available

Original:	J:36426 Toth LR, et al., Two distinct apolipoprotein B alleles in mice generated by a single 'in-out' targeting. Gene. 1996 31;178(1-2):161-8
All:	3 reference(s)