Probe:	Prtg cDNA1
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Uniform

Structure	Level	Pattern	Image	Note
TS12: primitive streak	Strong		1F
TS12: embryo	Present	Regionally restricted	1F	Expression is posterior.
TS12: future hindbrain	Present	Regionally restricted	1F	The anterior limit of expression is in the hindbrain.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	1G	Expression is absent in the ventral midline and the node.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted	1H	Expression is posterior.

Structure	Level	Pattern	Image	Note
TS13: future brain	Weak	Regionally restricted	3A	Expression is in the anterior neural plate, and in the neural tube adjacent to the first 5 somites.
TS13: future prosencephalon	Strong	Regionally restricted	3A	Expression is in the telencephalon.
TS13: rhombomere 4	Strong		3A
TS13: somite 01	Weak	Regionally restricted	3A
TS13: somite 02	Weak	Regionally restricted	3A
TS13: somite 03	Weak	Regionally restricted	3A
TS13: somite 04	Weak	Regionally restricted	3A
TS13: somite 05	Weak	Regionally restricted	3A
TS13: neural tube	Weak	Regionally restricted	3A	Expression is in the neural tube adjacent to the first 5 somites.
TS13: future spinal cord neural plate	Strong	Regionally restricted	3A	Expression is in the caudal neural plate.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	3B	Expression is in the telencephalon.
TS13: 1st branchial arch ectoderm	Present		3B
TS13: eye	Present		3B
TS13: somite 05	Present		3B
TS13: somite 06	Present		3B
TS13: somite 07	Present		3B
TS13: somite 08	Present		3B
TS13: somite 09	Present		3B
TS13: somite 10	Present		3B
TS13: neural tube	Present	Regionally restricted	3B	Expression is along the width of the caudal neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3C	Expression shifted posteriorly.
TS15: trunk somite	Present	Regionally restricted	3C	Expression shifted posteriorly.
TS15: branchial arch ectoderm	Strong		3C
TS15: head mesenchyme	Strong	Regionally restricted	3C	Expression is above the eye, as well as in the mesenchyme anterior to the eye.

Structure	Level	Pattern	Image	Note
TS13: future brain	Weak	Regionally restricted	3D	Expression is in the anterior neural plate.
TS13: future prosencephalon	Strong	Regionally restricted	3D	Expression is in the telencephalon.
TS13: rhombomere 4	Strong		3D
TS13: future spinal cord neural plate	Strong	Regionally restricted	3D	Expression is in the caudal neural plate.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Present	Regionally restricted	3E	Expression is in the telencephalon.
TS13: 1st branchial arch ectoderm	Present		3E
TS13: eye	Present		3E
TS13: rhombomere 4	Trace		3E
TS13: future spinal cord	Trace	Regionally restricted	3E	Expression is in the anterior spinal cord.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3F	In the spinal cord anterior to the forelimb, expression persisted in the most dorsal part of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3G	In the spinal cord anterior to the forelimb, expression persisted in the most dorsal part of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3H	From forelimb level (somites 8-12) and posteriorly, the labeling was detected in the entire width of the neural tube.
TS15: trunk dermomyotome	Present		3H

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present		3I

Structure	Level	Pattern	Image	Note
TS16: neural tube	Absent