Smurf1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:97483 Shi W, et al., Overexpression of Smurf1 negatively regulates mouse embryonic lung branching morphogenesis by specifically reducing Smad1 and Smad5 proteins. Am J Physiol Lung Cell Mol Physiol. 2004 Feb;286(2):L293-300
Assay type:	RT-PCR
MGI Accession ID:	MGI:3664762
Gene symbol:	Smurf1
Gene name:	SMAD specific E3 ubiquitin protein ligase 1

Probe:	Smurf1-pA, Smurf1-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative real-time PCR and is presented as the relative ratio to the level of Smurf1 mRNA at embryonic day 11.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (b)	Amount	Genetic Background	Mutant Allele(s)	Sex
E11	E11.0 (a)	TS18: lung	Weak	2 µg; total RNA	Swiss Webster		Not Specified
E14	E14.0 (a)	TS22: lung	Present	2 µg; total RNA	Swiss Webster		Not Specified
E16	E16.0 (a)	TS24: lung	Present	2 µg; total RNA	Swiss Webster		Not Specified
E18	E18.0 (a)	TS26: lung	Present	2 µg; total RNA	Swiss Webster		Not Specified
P1	P1	TS27: lung	Strong	2 µg; total RNA	Swiss Webster		Not Specified
Adult	P adult	TS28: lung	Present (c)	2 µg; total RNA	Swiss Webster		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) A gradual increase in expression up to P1.
(c) Expression in mouse adult lung was reduced by 45% compared with the level at P1 stage.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/30/2024 MGI 6.23