Probe:	Sim1 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS19: nephric duct	Present		3A
TS19: ureteric bud	Present	Regionally restricted	3A	Expression is throughout the ureteric epithelium and is enriched in newly formed tips.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	3B	Expression is at the tips.

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present	Regionally restricted	3B inset	Expression is enriched in the tips and is reduced in the trunk.

Structure	Level	Pattern	Image	Note
TS23: pretubular aggregate	Present		3C	Expression is in the pretubular aggregate/renal vesicle with very low expression in the tips.
TS23: renal vesicle	Present		3C	Expression is in the pretubular aggregate/renal vesicle with very low expression in the tips.
TS23: ureteric tip	Present		3C	Expression is in the pretubular aggregate/renal vesicle with very low expression in the tips.
TS23: maturing glomerular tuft	Present		3C
TS23: medullary renal tubule	Present		3C
TS23: early nephron	Present	Regionally restricted	3C	Expression is in comma- and S-shaped bodies.

Structure	Level	Pattern	Image	Note
TS23: ureteric tip	Trace		3C inset	Expression is low to absent at the tips.