Gene Expression Data
Assay Details
Assay
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Reference:
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J:112220
Caruana G, et al., Spatial gene expression in the T-stage mouse metanephros. Gene Expr Patterns. 2006 Dec;6(8):807-25
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3664484
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Gene symbol:
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Sim1
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Gene name:
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single-minded family bHLH transcription factor 1
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Probe:
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Sim1 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
Genetic Background:
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either: CD-1 or (involves: C57BL/6 * CBA)
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: nephric duct |
Present |
|
3A
|
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TS19: ureteric bud |
Present |
Regionally restricted |
3A
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Expression is throughout the ureteric epithelium and is enriched in newly formed tips.
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Specimen
3B:
embryonic day 12.5
(more )
Specimen
3B:
(close )
Genetic Background:
|
either: CD-1 or (involves: C57BL/6 * CBA)
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Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: metanephros |
Present |
Regionally restricted |
3B
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Expression is at the tips.
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Specimen
3B inset:
embryonic day 12.5
(more )
Specimen
3B inset:
(close )
Genetic Background:
|
either: CD-1 or (involves: C57BL/6 * CBA)
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: metanephros |
Present |
Regionally restricted |
3B inset
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Expression is enriched in the tips and is reduced in the trunk.
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
Genetic Background:
|
either: CD-1 or (involves: C57BL/6 * CBA)
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Age:
|
embryonic day 15.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Specimen
3C inset:
embryonic day 15.5
(more )
Specimen
3C inset:
(close )
Genetic Background:
|
either: CD-1 or (involves: C57BL/6 * CBA)
|
Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
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