Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Glra2tm1Clc
Name:	glycine receptor, alpha 2 subunit; targeted mutation 1, Constance L Cepko
MGI ID:	MGI:3662671
Gene:	Glra2  Location: ChrX:163912013-164110389 bp, - strand  Genetic Position: ChrX, 76.75 cM
Alliance:	Glra2tm1Clc page

Germline Transmission:	Earliest citation of germline transmission: J:111414
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intragenic deletion   Mutation details: A vector was designed to replace exons 6 and 7 with an FRT-flanked PGK-neomycin cassette. These exons encode two transmembrane domains and a large portion of its ligand binding domain. Alternative splicing of exon 5 with exon 8 would result in an immediate premature stop codon and should lead to a nonsense-mediated degradation of the mRNA. RT-PCR, Northern blot and in situ hybridization assays were performed to confirm absence of transcript in mutants. (J:111414)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Glra2 Mutation:	9 strains or lines available

Original:	J:111414 Young-Pearse TL, et al., Characterization of mice with targeted deletion of glycine receptor alpha 2. Mol Cell Biol. 2006 Aug;26(15):5728-34
All:	4 reference(s)