Probe:	Ox19.3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: cingulate cortex	Present	Regionally restricted	3-1b	Expression is present in the cingulate gyrus.

Structure	Level	Pattern	Image	Note
TS28: hippocampus	Present		3-2b

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Present		3-3b

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3-4b

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Present		5A	Expression is seen around the telencephalic vesicle.
TS17: midbrain	Present		5A	Expression is seen around the mesencephalic vesicle.
TS17: 1st branchial arch mandibular component	Present		5A
TS17: 3rd branchial pouch	Present		5A
TS17: 4th ventricle	Present		5A

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		5C
TS17: heart atrium	Present		5C
TS17: hindlimb bud	Present		5C	Expression is present in the left hindlimb bud.
TS17: neural tube	Present	Regionally restricted	5C	Expression present in present in the caudal end of the neural tube in the neuroepithelium.
TS17: ear	Present	Regionally restricted	5C	Expression is present around the cochlea.

Structure	Level	Pattern	Image	Note
TS20: embryo	Weak			There is a low level of diffuse expression in many tissues. There is also staining in the corpus striatum mediale.
TS20: hindbrain roof	Present
TS20: lateral ventricle	Present
TS20: cerebral cortex	Present	Regionally restricted		Expression is present in the roof of the neopallial cortex.
TS20: telencephalon	Present			Expression is present in the corpus striatum mediale.

Structure	Level	Pattern	Image	Note
TS23: embryo	Ambiguous			There is a low, diffuse signal throughout the embryo, but it may not be specific.