Summary | Transgene origin | Transgene description | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Tg(YACW408A5)1952Ricc
Name:	transgene insertion 1952, Andrea Riccio
MGI ID:	MGI:3655816
Synonyms:	YAC W408A5
Transgene:	Tg(YACW408A5)1952Ricc  Location: unknown
Alliance:	Tg(YACW408A5)1952Ricc page

Strain of Origin:

129/Sv

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Tg(YACW408A5)1952Ricc expresses 4 genes Transgene expresses: Organism Expressed Gene Note mouse Cdkn1c (MGI:104564) mouse Kcnq1 (MGI:108083) mouse Phlda2 (MGI:1202307) mouse Slc22a18 (MGI:1336884)   Mutation details: An 800kb yeast artificial chromosome (YAC) designated W408A5, which contained C57BL/6 distal chromosome 7 sequence from the Cars to Th gene, and including imprinting center(IC2 which contains Cdkn1c (P57) and Kcnq1) and is syntenic to human chromosome 11p15.5, was modified by addition of 2 copies of the Neo-selection cassette. The construct was introduced into 129/Sv ES cells by yeast spheroplast fusion. After maternal transmission of the transgene, most maternally expressed genes of the IC2 region (Phdla2, Slc22a18, Cdkn1c and Kcnq1) show 30-60% increased expression in the placenta relative to wild-type and equivalent or slightly reduced expression after paternal transmission. With paternal transmission, paternally expressed gene Kcnq1ot1 shows 2-fold increased expression over wild-type or after maternal transmission. Ascl2 expression (maternally expressed) is increased 2-fold after maternal or paternal transmission, while Tssc4 expression is increased 2-fold after maternal and by ~33% after paternal transmission. Imprinting of these 2 genes is not reproduced by the transgene. Expression of Cdkn1c and Kcnq1ot1 in the embryo is comparable to the placental expression (J:66461, J:96366)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:96366 Cerrato F, et al., The two-domain hypothesis in Beckwith-Wiedemann syndrome: autonomous imprinting of the telomeric domain of the distal chromosome 7 cluster. Hum Mol Genet. 2005 Feb 15;14(4):503-11
All:	2 reference(s)