Probe:	pT7T3Pac-mFrodo
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo mesoderm	Present		2B

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Strong		2C
TS10: embryo mesoderm	Strong	Regionally restricted	2C	Expression is present in the mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	2E	Expression is present in the caudal neuroectoderm.
TS12: primitive streak	Strong		2E
TS12: embryo mesenchyme	Strong	Regionally restricted	2E	Expression is present in the mesoderm adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS12: splanchnopleure	Present		2F	Expression is present in the splanchnic mesoderm.
TS12: embryo mesenchyme	Present		2F
TS12: cardiogenic plate	Weak		2F

Structure	Level	Pattern	Image	Note
TS12: intraembryonic coelom	Present		2G	Expression is present in the coelomic channel.
TS12: embryo mesenchyme	Present		2G

Structure	Level	Pattern	Image	Note
TS12: neural ectoderm	Present	Regionally restricted	2H	Expression is present in the caudal neuroectoderm and a subset of rostral neuroectoderm adjacent to the head folds.
TS12: embryo mesenchyme	Present		2H

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted		Although there is expression elsehwere in the embryo, there is none in the epiblast or the head folds.

Structure	Level	Pattern	Image	Note
TS12: embryo	Present	Regionally restricted		Although there is expression elsewhere in the embryo, there is none in the epiblast or in the head folds.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		3A
TS13: trunk unsegmented mesenchyme	Present		3A	Expression is present in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: somite	Present		3B

Structure	Level	Pattern	Image	Note
TS13: somite	Present		3C

Structure	Level	Pattern	Image	Note
TS13: facial neural crest	Present	Regionally restricted	3D	Expression is present in migrating neural crest; low levels are present in premigratory neural crest.
TS13: head mesenchyme	Present		3D
TS13: migrating neural crest	Present		3D

Structure	Level	Pattern	Image	Note
TS13: facial neural crest	Present		3F	Expression is present in migrating neural crest; low levels are present in premigratory neural crest.
TS13: head mesenchyme	Present		3F
TS13: migrating neural crest	Present		3F

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		3G	Expression is present in the presomitic mesoderm.
TS13: neural ectoderm	Present		3G

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present		3H
TS13: somite	Present		3H
TS13: head mesenchyme	Present		3H' , 3H
TS13: migrating neural crest	Present		3H , 3H'	Expression is present in migrating neural crest.

Structure	Level	Pattern	Image	Note
TS13: septum transversum	Present		3I	Expression is present in the septum transversum, including in the proepicardial organ and hepatic diverticulum.
TS13: neural ectoderm	Present		3I
TS13: somite	Present		3I

Structure	Level	Pattern	Image	Note
TS15: septum transversum	Present		3J	Expression is present in the septum transversum, including in the proepicardial organ and hepatic diverticulum.
TS15: trunk somite	Present	Graded	3J	Expression is distributed in a graded manner, being stronger caudally and being mainly restricted to the ventral-posterior region of each somite.
TS15: tail unsegmented mesenchyme	Present		3J	Expression is present in the presomitic mesoderm.
TS15: head mesenchyme	Present		3J
TS15: neural crest	Present		3J	Expression is present in the neural crest.

Structure	Level	Pattern	Image	Note
TS15: trunk somite	Present		3K	Expression is distributed in a graded manner, being stronger caudally and being mainly restricted to the ventral-posterior region of each somite.
TS15: tail unsegmented mesenchyme	Present		3K	Expression is present in the presomitic mesoderm.