Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:79830
Garel S, et al., The early topography of thalamocortical projections is shifted in Ebf1 and Dlx1/2 mutant mice. Development. 2002 Dec;129(24):5621-34
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3629736
|
|
Gene symbol:
|
Sema6a
|
|
Gene name:
|
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
|
|
Probe:
|
Sema6a probe1
|
|
Probe preparation:
|
Antisense RNA
|
|
Assay notes:
|
Because they have a have a wild type phenotype, heterozygous animals were used for controls.
|
|
|
Results
|
Specimen
5M:
embryonic day 16.5; Ebf1tm1Rug/Ebf1 +
(more  )
Specimen
5M:
(close  )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1+
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
5M
|
Expression is in the dorsal thalamus, low in medial nuclei, and is absent in the dorsal lateral geniculate nucleus.
|
Specimen
5N:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1tm1Rug
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: thalamus |
Present |
Regionally restricted |
5N
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
6I:
embryonic day 13.5
(more )
Specimen
6I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: thalamus mantle layer |
Present |
|
6I
|
|
| TS21: ganglionic eminence |
Present |
Regionally restricted |
6I
|
Expression is in a group of cells in the mantle of the ganglionic eminences and in the ventral pallium.
|
| TS21: telencephalon |
Present |
Regionally restricted |
6I
|
Expression is in a group of cells in the mantle of the ganglionic eminences and in the ventral pallium.
|
Specimen
6J:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1tm1Rug
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6K:
embryonic day 15.5
(more )
Specimen
6K:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
6L:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1tm1Rug
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: thalamus |
Present |
|
6L
|
Expression in this tissue is the same as in wild-type embryos.
|
| TS23: telencephalon |
Weak |
Regionally restricted |
6L
|
Expression is reduced in the basal ganglia.
|
Specimen
Not shown, WT:
embryonic day 14.5; Ebf1tm1Rug/Ebf1 +
(more )
Specimen
Not shown, WT:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
Not shown, -/-:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Ebf1tm1Rug/Ebf1tm1Rug
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: thalamus |
Present |
|
|
Expression in this mutant is the same as in wild-type embryos.
|
|
Analysis Tools