Antibody:	Anti-TACC1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS16: embryo	Present		1A	Ubiquitous expression.
TS16: branchial arch	Strong		1A
TS16: diencephalon lateral wall ventricular layer	Strong		1A
TS16: future hindbrain ventricular layer	Strong		1A
TS16: future midbrain ventricular layer	Strong		1A
TS16: liver	Strong		1A
TS16: telencephalon ventricular layer	Strong		1A

Structure	Level	Pattern	Image	Note
TS18: embryo	Present		1B	Ubiquitous expression.
TS18: brain ventricular layer	Strong		1B
TS18: branchial arch	Strong		1B
TS18: neural tube ventricular layer	Strong		1B

Structure	Level	Pattern	Image	Note
TS20: embryo	Present		1C	Ubiquitous expression.
TS20: brain	Strong		1C
TS20: dorsal root ganglion	Strong		1C
TS20: lung	Strong		1C
TS20: spinal cord	Strong		1C

Structure	Level	Pattern	Image	Note
TS21: embryo	Present		1D	Ubiquitous expression.
TS21: brain	Strong		1D	The highest expression is maintained in actively dividing tissues and neuronal progenitors prior to terminal differentiation.
TS21: spinal cord	Strong		1D	The highest expression is maintained in actively dividing tissues and neuronal progenitors prior to terminal differentiation.
TS21: dorsal root ganglion	Strong		1D
TS21: lung	Strong		1D

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1E	Ubiquitous expression.
TS22: brain	Strong		1E	The highest expression is maintained in actively dividing tissues and neuronal progenitors prior to terminal differentiation.
TS22: spinal cord	Strong		1E	The highest expression is maintained in actively dividing tissues and neuronal progenitors prior to terminal differentiation.
TS22: corpus striatum	Strong		1E
TS22: dorsal root ganglion	Strong		1E
TS22: lung	Strong		1E
TS22: midbrain	Strong		1E
TS22: neural retinal epithelium	Strong		1E
TS22: spinal cord ventricular layer	Strong		1E
TS22: cerebral cortex	Strong	Regionally restricted	1E	Expression is in the roof.
TS22: tail spinal cord	Strong	Regionally restricted	1E	Expression is in the ventricular zone.
TS22: cranial ganglion	Moderate		1E
TS22: salivary gland	Present		1E

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Graded	2A , 2B	The ependymal layer and alar plate exhibit the highest staining with decreasing expression toward the mantle layer.
TS14: future spinal cord	Present	Graded	2A , 2B	The ependymal layer and alar plate exhibit the highest staining with decreasing expression toward the mantle layer.
TS14: notochord	Present		2B , 2A
TS14: nervous system	Moderate	Regionally restricted	2A , 2B	Expression is in dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Present	Regionally restricted	2C , 2D	Expression is in proliferating cells.
TS16: 1st branchial arch	Present	Regionally restricted	2D , 2C	Expression is in the epithelium.

Structure	Level	Pattern	Image	Note
TS20: brain	Present		2E
TS20: choroid plexus	Present		2E

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		2F
TS20: vertebral cartilage condensation	Present	Single cells	2F	Expression is in a chondrocyte.

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Moderate		2G	Cytoplasmic staining.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present		2H	Glial cells have nuclear staining.

Structure	Level	Pattern	Image	Note
TS14: heart	Strong	Regionally restricted	3A	Expression is in myogenic precursors.

Structure	Level	Pattern	Image	Note
TS16: outflow tract	Present	Regionally restricted	3B	Expression is in the epithelial layers.
TS16: heart	Present	Regionally restricted	3B	Expression is in the cardiac wall.

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Weak		3C
TS21: dorsal aorta	Strong		3C
TS21: outflow tract	Strong		3C
TS21: pulmonary artery	Strong		3C
TS21: liver	Present		3C
TS21: lung	Present		3C

Structure	Level	Pattern	Image	Note
TS21: dorsal aorta	Strong	Regionally restricted	3D	Expression is particularly strong in the adventitia and endothelium.
TS21: pulmonary artery	Strong	Regionally restricted	3D	Expression is particularly strong in the adventitia and endothelium.

Structure	Level	Pattern	Image	Note
TS24: aorta	Strong	Regionally restricted	3E , 3G , 3F	Expression is particularly strong in the adventitia and endothelium.
TS24: pulmonary trunk	Strong	Regionally restricted	3E , 3G , 3F	Expression is particularly strong in the adventitia and endothelium.
TS24: mitral valve	Strong	Regionally restricted	3G , 3E , 3F	Expression is the endothelium.
TS24: heart right ventricle	Strong	Regionally restricted	3E , 3F , 3G	Expression is in the outflow tract.
TS24: innominate artery	Strong		3G , 3F , 3E
TS24: lung	Strong		3G , 3F , 3E
TS24: thymus	Strong		3G , 3F , 3E
TS24: trachea	Strong		3G , 3F , 3E

Structure	Level	Pattern	Image	Note
TS18: Rathke's pouch	Very strong		4A
TS18: branchial arch	Present		4A

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Strong		4B	Staining is cytoplasmic.
TS21: lung mesenchyme	Strong		4B	Staining is nuclear.

Structure	Level	Pattern	Image	Note
TS21: stomach epithelium	Very strong		4C , 4D	Staining is cytoplasmic.
TS21: stomach mesenchyme	Very strong		4C , 4D	Staining is nuclear.
TS21: liver	Present		4C , 4D

Structure	Level	Pattern	Image	Note
TS22: stomach epithelium	Very strong		4F , 4E	Staining is cytoplasmic.
TS22: stomach mesenchyme	Very strong		4F , 4E	Staining is nuclear.
TS22: liver	Present		4F , 4E

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Very strong		4G	Staining is cytoplasmic.
TS24: oral region	Present	Regionally restricted	4G	Nuclear staining in the mesenchyme underlying the epithelium.

Structure	Level	Pattern	Image	Note
TS24: esophagus epithelium	Present		4H	Staining is cytoplasmic.
TS24: esophagus mesenchyme	Very strong	Regionally restricted	4H	Staining is nuclear in the muscle cells of the muscularis propria.
TS24: liver	Present		4H

Structure	Level	Pattern	Image	Note
TS24: intestine epithelium	Present		4I	Staining is cytoplasmic.
TS24: intestine mesenchyme	Present		4I	Staining is nuclear in the mucosal layer and the muscularis propria.

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Present	Regionally restricted	4J	Expression is in serous cells and the encapsulating myoepithelium.

Structure	Level	Pattern	Image	Note
TS24: pancreas	Present	Regionally restricted	4K	Expression is at the end of the branching glandular components of the exocrine pancreas.

Structure	Level	Pattern	Image	Note
TS24: thymus	Present		4L

Structure	Level	Pattern	Image	Note
TS24: pharyngo-tympanic tube	Strong
TS24: metanephros	Present

Structure	Level	Pattern	Image	Note
TS28: salivary gland	Present