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Gene Expression Data
Assay Details
Assay
Reference: J:107770 Nakamura K, et al., Endogenous and exogenous glucocorticoid regulation of ENaC mRNA expression in developing kidney and lung. Am J Physiol Cell Physiol. 2002 Sep;283(3):C762-72
Assay type: RNase protection
MGI Accession ID: MGI:3625184
Gene symbol: Scnn1a
Gene name: sodium channel, nonvoltage-gated 1 alpha
Probe: alpha-mENaC cDNA6
Probe preparation: Antisense labelled with biotin RNA
Visualized with: Autoradiography
Assay notes: Shown is a graph of the quantitative analysis. Relative abundance of mENaC for CRH knock-out and wild-type mice normalized to 18S RNA and to the abundance at E18.5.
Results Image: 2_alpha lung
 Sample Information Bands Other Sample Information
Lane AgeStructure 125 b (a) Amount Genetic BackgroundMutant Allele(s)Sex
wt Lung E16.5 TS24: lung Present 25 µg; total RNA Not Specified Not Specified
wt Lung E17.5 TS25: lung Present 25 µg; total RNA Not Specified Not Specified
wt Lung E18.5 TS26: lung Present 25 µg; total RNA Not Specified Not Specified
CRH-KO Lung E16.5 TS24: lung Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
CRH-KO Lung E17.5 TS25: lung Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
CRH-KO Lung E18.5 TS26: lung Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
Notes:
(a) In both mutant and wild-type embryos there is an increase in expression with increasing age.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory