Antibody:	pan-espin antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: endolymphatic appendage	Present		1K	Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.
TS17: inner ear	Present		1K	Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.
TS17: otocyst	Present		1K	Expression is present in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), and the clefts between branchial arches.

Structure	Level	Pattern	Image	Note
TS17: endolymphatic appendage	Absent		1L	Unlike in the heterozygote, there is no expression in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), or in the clefts between branchial arches.
TS17: inner ear	Absent		1L	Unlike in the heterozygote, there is no expression in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), or in the clefts between branchial arches.
TS17: otocyst	Absent		1L	Unlike in the heterozygote, there is no expression in the otic pit, the otocyst, the developing membranous labyrinth (including endoymphatic duct, vertical canal pouch, anterior and posterior semicircular canal), or in the clefts between branchial arches.