Probe:	pBSSac400
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors state that the in situ analysis with this probe gave identical results as the data shown with probe pBSNsk2K (MGI:25635), which is shown in assay: MGI:3623505.

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Absent
TS13: somite	Absent

Structure	Level	Pattern	Image	Note
TS17: trunk dermomyotome	Present
TS17: cardiac muscle tissue	Absent

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted
TS20: facial ganglion	Present	Regionally restricted
TS20: cardiac muscle tissue	Absent

Structure	Level	Pattern	Image	Note
TS22: forelimb musculature	Present			The higher magnification shows expression in myofibers.
TS22: skeletal muscle	Present			The higher magnification shows expression in myofibers.
TS22: humerus cartilage condensation	Present	Regionally restricted		Expression is in sites of ossification in the periosteal layer of develpoing bones.
TS22: rib cartilage condensation	Present	Regionally restricted		Expression is in sites of ossification in the periosteal layer of develpoing bones.
TS22: vertebral cartilage condensation	Present	Regionally restricted		Expression is in sites of ossification in the periosteal layer of develpoing bones.
TS22: dermis	Present
TS22: dorsal root ganglion	Present
TS22: maturing glomerular tuft	Present
TS22: thymus primordium	Present
TS22: trachea epithelium	Present
TS22: lung	Present	Regionally restricted		Expression is in segmented bronchi and terminal bronchioles.
TS22: enteric nervous system	Present	Regionally restricted		Expression is in enteric ganglia of the gut.
TS22: intestine epithelium	Present	Regionally restricted		Expression is in the secretory epithelia.
TS22: vertebral pre-cartilage condensation	Absent