About   Help   FAQ
Spi1tm1.2Dgt
Targeted Allele Detail
Summary
Symbol: Spi1tm1.2Dgt
Name: Spi-1 proto-oncogene; targeted mutation 1.2, Daniel G Tenen
MGI ID: MGI:3619527
Synonyms: -14kb UREloxP (neo-), URE floxed (neo-)
Gene: Spi1  Location: Chr2:90912750-90946104 bp, + strand  Genetic Position: Chr2, 50.44 cM, cytoband E3
Alliance: Spi1tm1.2Dgt page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:106788
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsThis allele was generated by crossing mice bearing Sfpi1tm1Dgt with mice ubiquitously expressing FLP recombinase to produce progeny in which the FRT-flanked PGKneo cassette has been deleted. This leaves a single FRT site immediately upstream of the 5' member of a pair of loxP sites flanking the 3.4-kb upstream regulatory element (URE) that is located 14 kb upstream of the endogenous gene. Expression of the protein is unaffected by presence of the FRT or loxP sites. (J:90331, J:106788)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Spi1 Mutation:  27 strains or lines available
Notes
Crossing mice bearing this "floxed" allele with mice expressing Cre recombinase under control of a selected promoter will result in excision of the URE in the cell/tissue type of interest.
References
Original:  J:106788 Zhang P, Sfpi1tm1.2 (URE loxP [neo-]) development. Personal Communication. 2006;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
04/16/2024
MGI 6.23
The Jackson Laboratory