Antibody:	Rif1 antibody
Detection system:	Secondary antibody
Assay notes:	Fixative was 3.7% formaldehyde in 0.1M potassium phosphate. Some ages were assigned by curator based on morphological criteria supplied by authors.

Structure	Level	Pattern	Image	Note
TS28: germ cell of ovary	Present		3A	Expression is in the nucleus of the cell but is excluded from the nucleolus.

Structure	Level	Pattern	Image	Note
TS28: germ cell of ovary	Present	Uniform	3B

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		3C	Expression is in the pronuclei.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		3D	Expression is in the pronuclei.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Present		3E	Expression is nuclear.
TS2: second polar body	Absent		3E

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Present		3F	Expression is nuclear.
TS3: second polar body	Absent		3F

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		3G	Expression is nuclear.
TS3: second polar body	Absent		3G

Structure	Level	Pattern	Image	Note
TS3: compacted morula	Present		3H	Expression is nuclear.
TS3: second polar body	Absent		3H

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Absent		3I
TS5: mural trophectoderm	Present		3I	Expression is in the nuclei.
TS5: polar trophectoderm	Present		3I	Expression is in the nuclei.

Structure	Level	Pattern	Image	Note
TS6: trophectoderm	Present		3J
TS6: epiblast	Absent		3J
TS6: primitive endoderm	Absent		3J

Structure	Level	Pattern	Image	Note
TS8: epiblast	Strong		4_5.5 dpc

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present		4_6.5 dpc

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		4_7.5 dpc
TS11: Reichert's membrane	Weak		4_7.5 dpc
TS11: embryo mesoderm	Absent		4_7.5 dpc
TS11: primordial germ cell	Absent		4_7.5 dpc

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		4_8.5 dpc
TS13: primordial germ cell	Absent		4_8.5 dpc

Structure	Level	Pattern	Image	Note
TS15: hindgut epithelium	Present	Single cells	4_9.5 dpc	Expression is in some cells in the hindgut epithelium.
TS15: primordial germ cell	Present	Single cells	4_9.5 dpc

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5_11.5 dpc male mRif1

Structure	Level	Pattern	Image	Note
TS21: germ cell of testis	Present		5_13.5 dpc male mRif1

Structure	Level	Pattern	Image	Note
TS23: germ cell of testis	Weak		5_15.5 dpc male mRif1

Structure	Cell Type	Level	Pattern	Image	Note
TS25: Sertoli cell	Sertoli cell	Weak		5_17.5 dpc male mRif1

Structure	Cell Type	Level	Pattern	Image	Note
TS27: Sertoli cell	Sertoli cell	Weak		5_1 dpp male mRif1	Low in Sertoli cells, absent in germ cells.
TS27: germ cell of testis		Absent		5_1 dpp male mRif1	Low in Sertoli cells, absent in germ cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Sertoli cell	Sertoli cell	Present		5_5 dpp male mRif1
TS28: germ cell of testis		Absent		5_5 dpp male mRif1

Structure	Cell Type	Level	Pattern	Image	Note
TS28: germ cell of testis		Present		5_10 dpp male mRif1	Expression is in all the male germ cells toward the periphery of the testis cords at this stage but is absent from the more strongly GCNA-positive zygotene spermatocytes towards the center.
TS28: Sertoli cell	Sertoli cell	Present		5_10 dpp male mRif1

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell of gonad	Present		5_11.5 dpc female mRif1

Structure	Level	Pattern	Image	Note
TS21: germ cell of ovary	Present		5_13.5 dpc female mRif1

Structure	Level	Pattern	Image	Note
TS23: germ cell of ovary	Weak		5_15.5 dpc female mRif1

Structure	Level	Pattern	Image	Note
TS25: germ cell of ovary	Weak		5_17.5 dpc female mRif1
TS25: primary oocyte	Weak		5_17.5 dpc female mRif1

Structure	Level	Pattern	Image	Note
TS27: primary oocyte	Weak		5_1 dpp female mRif1

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		5_5 dpp female mRif1

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Strong		5_10 dpp female mRif1	Expression is stronger in growing oocytes in primary follicles than in nongrowing oocytes in primordial follicles.

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6A	Expression is in cells located at the periphery of the testis cords.

Structure	Level	Pattern	Image	Note
TS28: testis	Present	Regionally restricted	6B	Expression is in cells located at the periphery of the testis cords.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		6C	Expression is in the nuclei of oocytes.
TS28: ovary follicle	Weak		6C

Structure	Level	Pattern	Image	Note
TS28: oocyte of primordial follicle	Present		6D	Expression is in the nuclei of oocytes in primordial follicles.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		6E	Expression is in the nuclei of growing oocytes in growing follicles.

Structure	Level	Pattern	Image	Note
TS28: primary oocyte	Present		6F	Expression is in the nuclei of growing oocytes in growing follicles.

Structure	Level	Pattern	Image	Note
TS28: oocyte of mature follicle	Present		6G	Expression is in the nuclei of fully grown oocytes in antral follicles.