Probe:	IMAGE clone 718668
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS17: arterial system	Weak	Regionally restricted	4C	Expression is present in the aortic sac.
TS17: 1st branchial arch mesenchyme	Weak		4C
TS17: septum transversum	Present	Regionally restricted	4C	Expression is present in a small population of cells in lateral regions of the septum transversum.

Structure	Level	Pattern	Image	Note
TS20: pulmonary artery	Strong	Regionally restricted	4H	Expression is found in the endothelial cells in the pulmonary artery.

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Weak		5J	Expression is present in lateral mesoderm.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present		5M	Expression is present in mesenchymal cells around and between mesoderm cells condensing to form bone and muscle. It is absent from medial regions of developing bone.

Structure	Level	Pattern	Image	Note
TS20: limb mesenchyme	Present	Regionally restricted	5P	Expression is present in mesenchymal cells around and between mesoderm cells condensing to form bone and muscle. It is absent from medial regions of developing bone.

Structure	Level	Pattern	Image	Note
TS20: nose	Present	Regionally restricted	6C	Expression is present in mesoderm derived cells in the nasal capsule.
TS20: cartilage	Present		6C
TS20: primary palate	Present		6C
TS20: head mesenchyme	Present		6C	Expression is present in almost all head mesoderm cells. There is weak expression adjacent to hair or whisker follicles and strong expression around the neural epithelium, optic stalk and eye, facial ganglia, developing ear and lobes and surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: primary palate	Strong	Regionally restricted	6G	There is strong expression in the developing palatal mesoderm.
TS20: head mesenchyme	Present		6G	Expression is present in almost all head mesoderm cells. There is weak expression adjacent to tooth primordia and strong expression around the neural epithelium, optic stalk and eye, facial ganglia, developing ear and earlobes and surface ectoderm.
TS20: vibrissa	Weak		6G

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	8B	Expression is present in the ventral diencephalon.
TS17: midbrain	Present	Regionally restricted	8B	Expression is present in the cranial flexure.

Structure	Level	Pattern	Image	Note
TS19: eye	Present	Regionally restricted	8E	Expression is present in ventral eyelid cells.
TS19: cornea	Strong		8E
TS19: embryonic fissure	Present		8E
TS19: head mesenchyme	Present	Regionally restricted	8E	Expression is present in mesenchymal cells condensing around the eye and the optic track.

Structure	Level	Pattern	Image	Note
TS19: eye	Present	Regionally restricted		Expression is present in developing ocular muscles.

Structure	Level	Pattern	Image	Note
TS20: pericardial component	Strong			Strong expression is present in the pericardial sac.
TS20: intraembryonic coelom	Present	Regionally restricted		Expression is present in the mesenchyme of the developing diaphragm.
TS20: peritoneal component	Present
TS20: hypothalamus ventricular layer	Present
TS20: spinal cord	Absent

Structure	Level	Pattern	Image	Note
TS23: embryo mesenchyme	Trace			Expression is barely detectable in undifferentiated lateral mesenchymal cells in the body and limbs.
TS23: limb mesenchyme	Trace			Expression is barely detectable in undifferentiated lateral mesenchymal cells in the body and limbs.

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expression is present in the medial and lateral mesoderm of the maternal decidua.

Structure	Level	Pattern	Image	Note
TS16: heart	Present			Expression is present in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: heart	Present			Expression is present in the endothelium.

Structure	Level	Pattern	Image	Note
TS23: skeleton	Absent