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Gene Expression Data
Assay Details
Assay
Reference: J:3969 Zhou X, et al., Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. Nature. 1993 Feb 11;361(6412):543-7
Assay type: RT-PCR
MGI Accession ID: MGI:3589366
Gene symbol: Nodal
Gene name: nodal growth differentiation factor
Probe: P1133, RC-9
Assay notes: Three rounds of fully nested PCR were used; the first set of primers used was NH-2 and C6 (MGI:3589359) and the second set used was P1112 and P1447 (MGI:3589361).
Results
Image: 3 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 290 bp Amount Genetic BackgroundMutant Allele(s)Sex
1 Mol. Wt. Marker Lane
2 E6.25 (a) TS9: embryo Present 1 µg; total RNA (ICR x Swiss Webster)F1 Not Specified
3 E6.75 (b) TS10: embryo Present 1 µg; total RNA (ICR x Swiss Webster)F1 Not Specified
4 E7.25 (c) TS10: embryo Present 1 µg; total RNA (ICR x Swiss Webster)F1 Not Specified
5 E7.5 (d) TS11: embryo Present 1 µg; total RNA (ICR x Swiss Webster)F1 Not Specified
6 Control
7 Mol. Wt. Marker Lane
Notes:
(a) The embryos used for this preparation were at pre- and very early streak stage.
(b) The embryos used for this preparation were at early streak stage.
(c) The embryos used for this preparation were at late streak stage but before appearance of the node.
(d) The embryos used for this preparation were at the head fold stage with the node visible.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory