Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1.1(birA)Mejr
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dies Meijer
MGI ID:	MGI:3588507
Synonyms:	bira, Rosa26BirA
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1.1(birA)Mejr page

Germline Transmission:	Earliest citation of germline transmission: J:100227
Parent Cell Line:	IB10/E14IB10 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Not Applicable) Mutation:    Insertion   Mutation details: An HA (hemagglutinin) tagged E. coli bacterial birA, bifunctional protein (biotin ligase), gene was inserted into exon 3 of the rabbit beta-globin gene. The entire birA ORF, with the rabbit beta globin splice acceptor and transcription termination sequences were excised to generate a targeting vector that also contained a floxed PGK- puromycin selection cassette. This targeting vector was inserted into the first intron of the Gt(ROSA)26Sor gene. Cre-mediated recombination removed the floxed puromycin cassette. Western blot of mutants demonstrated the expression of birA in all tissues tested, though levels of expression varied from tissue to tissue. (J:100227)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 10 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:100227 Driegen S, et al., A generic tool for biotinylation of tagged proteins in transgenic mice. Transgenic Res. 2005 Aug;14(4):477-82
All:	29 reference(s)