Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:99531
Benedito R, et al., Expression of Dll4 during mouse embryogenesis suggests multiple developmental roles. Gene Expr Patterns. 2005 Aug;5(6):750-5
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:3587944
|
|
Gene symbol:
|
Dll4
|
|
Gene name:
|
delta like canonical Notch ligand 4
|
|
Reporter:
|
lacZ
|
|
|
Direct Detection
|
|
Assay notes:
|
Embryos were fixed in 0.2% glutaraldehyde.
|
|
|
Results
|
Specimen
1A:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more  )
Specimen
1A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
Specimen
1B:
embryonic day 14.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
Specimen
1C:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
Specimen
1D:
embryonic day 9.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: yolk sac |
Present |
Regionally restricted |
1D
|
Expression is in the arteries.
|
Specimen
1E:
embryonic day 16.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: yolk sac |
Present |
Regionally restricted |
1E
|
Staining is in the arterioles and in all capillary beds, but is absent from the venules.
|
Specimen
1F:
postnatal adult; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
Specimen
1G:
postnatal adult; Dll4tm1Jrt/Dll4 +
(more )
Specimen
1G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
Glutaraldehyde
|
Specimen
2A:
embryonic day 9.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2A:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 9.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: neural tube lateral wall |
Present |
Regionally restricted |
2A
|
Expression is in neurons localized in the marginal zone, with higher and more internal expression in the ventral half of the neural tube.
|
Specimen
2B:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: blood vessel |
Present |
Regionally restricted |
2B
|
Expression is in blood vessels invading the neural tube.
|
| TS17: neural tube lateral wall |
Present |
Regionally restricted |
2B
|
Expression is in a defined stripe of neurons in the marginal zone, absent in the inner ventricular zone.
|
Specimen
2C:
embryonic day 11.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2F:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2G:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2G:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2H:
embryonic day 14.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2I:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2I:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2J:
embryonic day 13.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2J:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2K:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
2K:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: dorsal aorta |
Strong |
Regionally restricted |
2K
|
Expression is in para-aortic bodies that lie close to the main aorta.
|
Specimen
3A:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Absent |
|
3A
|
Expression is absent in the retina.
|
Specimen
3B:
embryonic day 11.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: retina |
Present |
Regionally restricted |
3B
|
Expression is in the region surrounding the optic nerve.
|
Specimen
3C:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3D:
embryonic day 14.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Graded |
3D
|
The outer layer has many positive cells, the intermediate layer has moderate expression and the ganglion and amacrine cell layer has few expressing cells.
|
Specimen
3E:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3F:
embryonic day 14.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 14.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Graded |
3F
|
The outer layer has many positive cells, the intermediate layer has moderate expression and the ganglion and amacrine cell layer has few expressing cells.
|
Specimen
3H:
embryonic day 16.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina |
Present |
Graded |
3H
|
The outer layer has many positive cells, the intermediate layer has moderate expression and the ganglion and amacrine cell layer has few expressing cells.
|
Specimen
3I:
postnatal adult; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3I:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3J:
embryonic day 10.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3J:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3K:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3K:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3L:
embryonic day 15.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3L:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3N:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3N:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
3O:
embryonic day 15.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
3O:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4A:
embryonic day 15.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
4A:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: intestine epithelium |
Present |
Regionally restricted |
4A
|
Expression is in epithelial cells of the villus and intervillus.
|
Specimen
4D:
embryonic day 12.5; Dll4tm1Jrt/Dll4 +
(more )
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * ICR
|
|
Mutant Allele(s):
|
Dll4tm1Jrt/Dll4+
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Glutaraldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools