Antibody:	GATA-3 antibody
Detection system:	Secondary antibody coupled to rhodamine
Assay notes:	The fixation was in methanol/acetone.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Uniform	1A , 1B	This area shows labeling in the basal turn.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct epithelium	Present	Uniform	1A , 1C	This area shows labeling in the apical turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	2B	This view is of the epithelium of the apical turn.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Weak	Regionally restricted	2D	This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Weak	Regionally restricted	2E	This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Present	Regionally restricted	3A	This view is or the basal turn. The outer hair cells show little label, but the inner hair cells show positive labeling. There is strong labeling in phalangeal cells, Deiter's cells, pillar cells and Hensen's cells.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Single cells	4B	In the basal turn, labeling intensity has decreased in all cells. This includes supporting cells in the organ of Corti, from the base to the apex of the cochlea. In the apical turn, hair cells were not labeled, while Deiter's cells remained positive.

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Absent		5A	Expression is absent in the entire epithelium.

Structure	Level	Pattern	Image	Note
TS24: cochlear duct epithelium	Present	Single cells	7B , 7A	The spiral ganglion cells are positively labeled. There is a decrease in the labeling of the hair cells in the basal turn that is described in the text.

Structure	Level	Pattern	Image	Note
TS24: utricle epithelium	Absent		8A , 8B

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Weak	Single cells		Only scattered cells remain positive.

Structure	Level	Pattern	Image	Note
TS23: cochlea	Present	Regionally restricted	6	Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E15.5.

Structure	Level	Pattern	Image	Note
TS22: cochlea	Present	Regionally restricted	6	Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E14.5.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present	Regionally restricted	6	Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 80% expressed Gata3 at E16.5.

Structure	Level	Pattern	Image	Note
TS26: cochlea	Weak	Regionally restricted	6	Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 30% expressed Gata3 at E18.5.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Weak	Regionally restricted	6	Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 15% expressed Gata3 at P0.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Absent		6	Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 0% expressed Gata3 at P14.