Il17b RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:94618 You Z, et al., Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF. Biochem Biophys Res Commun. 2005 Jan 21;326(3):624-31
Assay type:	RT-PCR
MGI Accession ID:	MGI:3525447
Gene symbol:	Il17b
Gene name:	interleukin 17B

Probe:	Il17b-pA, Il17b-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Real-time quantitative RT-PCR was used to obtain these results.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E10.5	E10.5 (a)	TS17: forelimb bud TS17: hindlimb bud	Weak	5 µg; total RNA	CD-1		Not Specified
E11.5	E11.5 (a)	TS19: forelimb bud TS19: hindlimb bud	Present (b)	5 µg; total RNA	CD-1		Not Specified
E12.5	E12.5 (a)	TS20: forelimb TS20: hindlimb	Present (c)	5 µg; total RNA	CD-1		Not Specified
E13.5	E13.5 (a)	TS21: forelimb TS21: hindlimb	Present (d)	5 µg; total RNA	CD-1		Not Specified
E14.5	E14.5 (a)	TS22: forelimb TS22: hindlimb	Present (e)	5 µg; total RNA	CD-1		Not Specified
E15.5	E15.5 (a)	TS23: forelimb TS23: hindlimb	Present (f)	5 µg; total RNA	CD-1		Not Specified
E16.5	E16.5 (a)	TS24: forelimb TS24: hindlimb	Present	5 µg; total RNA	CD-1		Not Specified
E17.5	E17.5 (a)	TS25: forelimb TS25: hindlimb	Present (g)	5 µg; total RNA	CD-1		Not Specified
E19.5	E19.5 (a)	TS26: forelimb TS26: hindlimb	Weak (h)	5 µg; total RNA	CD-1		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression levels are 4 fold what is seen at E10.5.
(c) Expression levels are 64 fold what is seen at E10.5.
(d) Expression levels are 256 fold what is seen at E10.5.
(e) This is the time of peak expression; expression levels are 588 fold what is seen at E10.5.
(f) Expression levels are 158 fold what is seen at E10.5.
(g) Expression levels are 104 fold what is seen at E10.5.
(h) Expression levels are 7 fold what is seen at E10.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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