Gene Expression Data
Assay Details
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Assay
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Reference:
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J:83696
Ford-Perriss M, et al., Localisation of specific heparan sulfate proteoglycans during the proliferative phase of brain development. Dev Dyn. 2003 Jun;227(2):170-84
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3522621
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Gene symbol:
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Hspg2
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Gene name:
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perlecan (heparan sulfate proteoglycan 2)
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Antibody:
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906
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
2A:
embryonic day 10.5
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forebrain |
Present |
Regionally restricted |
2A
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Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
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| TS17: hindbrain |
Present |
Regionally restricted |
2A
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Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
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| TS17: midbrain |
Present |
Regionally restricted |
2A
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Strong immunoreactivity in basement membrane surrounding neural tube and in basement membrane of overlying ectoderm; also in mesodermal matrix outside tube. No expression on neuroepithelial cells within tube, or in surrounding matrix.
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Specimen
2A':
embryonic day 10.5
(more )
Specimen
2A':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
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Section through dorsal telencephalon. Two lines of immunoreactivity define the basement membrane lining the neural tube and also the ectoderm.
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Specimen
2B,2B'':
embryonic day 10.5
(more )
Specimen
2B,2B'':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C,2C'':
embryonic day 10.5
(more )
Specimen
2C,2C'':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools