Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Phox2atm1(Phox2b)Mist
Name:	paired-like homeobox 2a; targeted mutation 1, Michele Studer
MGI ID:	MGI:3521825
Synonyms:	Phox2aKI2b
Gene:	Phox2a  Location: Chr7:101467520-101471933 bp, + strand  Genetic Position: Chr7, 54.66 cM
Alliance:	Phox2atm1(Phox2b)Mist page

Germline Transmission:	Earliest citation of germline transmission: J:126634
Parent Cell Line:	TBV2 (ES Cell)
Strain of Origin:	129S2/SvPas

Allele Type:    Targeted Mutation:    Insertion   Mutation details: The Phox2b coding sequence was inserted upstream of exon 1 along with a floxed neo cassette which was later removed by cre-mediated recombination following a cross to Tg(ACTB-cre)1Nagy mice. The insertion creates a fusion protein. Southern blot analysis and PCR genotyping were used to confirm the insertion. Immunohistochemistry was used to confirm the alteration in expression patterns. (J:126634)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Phox2a Mutation:	10 strains or lines available

Original:	J:126634 Coppola E, et al., Reciprocal gene replacements reveal unique functions for Phox2 genes during neural differentiation. EMBO J. 2005 Dec 21;24(24):4392-403
All:	1 reference(s)