Fgf2tm2Doe
Targeted Allele Detail
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| Symbol: |
Fgf2tm2Doe |
| Name: |
fibroblast growth factor 2; targeted mutation 2, Thomas Doetschman |
| MGI ID: |
MGI:3512180 |
| Synonyms: |
18-kDa FGF2KO, Fgf2 lmw ko |
| Gene: |
Fgf2 Location: Chr3:37402616-37464255 bp, + strand Genetic Position: Chr3, 18.41 cM, cytoband A2-B
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| Alliance: |
Fgf2tm2Doe page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:94550
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| Parent Cell Line: |
E14TG2a (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exchange reaction of a Tag & Exchange targeting scheme 4 bp alteration without introduction of marker gene or loxP or frt sites. To produce this ko allele, the Hprt minigene in the tagged Fgf2 ko allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a mutant exon 1 with the sequence CTGCAG replacing the wild-type CCATGC. The mutant sequence encodes an alanine GCA in place of the methionine ATG translational start site of the 18-kDa low molecular weight isoform. The adjacent upstream wobble base was also changed from C to T so that the composite of these three base changes generated a PstI site that is diagnostic for the exchanged allele. Cells with the exchanged allele were selected for the loss of HPRT function using 5 mg/mL 6-thioguanine. There are no LoxP, Frt or marker gene sequences in this new allele.
(J:94550)
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View phenotypes and curated references for all genotypes (concatenated display).
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E14TG2a congenic line with one Fgf2 ko allele was used to produce this allele.
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| Original: |
J:94550 Garmy-Susini B, et al., Role of fibroblast growth factor-2 isoforms in the effect of estradiol on endothelial cell migration and proliferation. Circ Res. 2004 May 28;94(10):1301-9 |
| All: |
11 reference(s) |
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Analysis Tools
