Vnn1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93500 Bouma GJ, et al., Using real time RT-PCR analysis to determine multiple gene expression patterns during XX and XY mouse fetal gonad development. Gene Expr Patterns. 2004 Nov;5(1):141-9
Assay type:	RT-PCR
MGI Accession ID:	MGI:3511041
Gene symbol:	Vnn1
Gene name:	vanin 1

Probe:	Vnn1-pA, Vnn1-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Real time RT-PCR was used for these analyses.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E10.5 XX	E11.0 (a)	TS18: gonad primordium TS18: mesonephros	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E10.5 XY	E11.0 (a)	TS18: gonad primordium TS18: mesonephros	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E11.5 XX	E12.0 (a)	TS20: mesonephros of female TS20: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E11.5 XY	E12.0 (a)	TS20: mesonephros of male TS20: testis	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E12.0 XX gonad	E12.5 (a)	TS20: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E12.0 XX mesonephros	E12.5 (a)	TS20: mesonephros of female	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E12.0 XY gonad	E12.5 (a)	TS20: testis	Present (b)	.1-.4 µg; total RNA	C57BL/6JEi		Male
E12.0 XY mesonephros	E12.5 (a)	TS20: mesonephros of male	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E12.5 XX gonad	E13.0 (a)	TS21: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E12.5 XX mesonephros	E13.0 (a)	TS21: mesonephros of female TS21: nephric duct of female TS21: paramesonephric duct of female	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E12.5 XY gonad	E13.0 (a)	TS21: testis	Present (b)	.1-.4 µg; total RNA	C57BL/6JEi		Male
E12.5 XY mesonephros	E13.0 (a)	TS21: mesonephros of male TS21: nephric duct of male TS21: nephric duct of male, mesonephric portion	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E13.0 XX gonad	E13.5 (a)	TS21: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E13.0 XX mesonephros	E13.5 (a)	TS21: mesonephros of female TS21: nephric duct of female TS21: paramesonephric duct of female	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E13.0 XY gonad	E13.5 (a)	TS21: testis	Present (b)	.1-.4 µg; total RNA	C57BL/6JEi		Male
E13.0 XY mesonephros	E13.5 (a)	TS21: mesonephros of male TS21: nephric duct of male TS21: nephric duct of male, mesonephric portion	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E13.5 XX gonad	E14.0 (a)	TS22: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E13.5 XX mesonephros	E14.0 (a)	TS22: mesonephros of female TS22: nephric duct of female TS22: paramesonephric duct of female	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E13.5 XY gonad	E14.0 (a)	TS22: testis	Present (b)	.1-.4 µg; total RNA	C57BL/6JEi		Male
E13.5 XY mesonephros	E14.0 (a)	TS22: mesonephros of male TS22: nephric duct of male TS22: nephric duct of male, mesonephric portion	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E14.0 XX gonad	E14.5 (a)	TS22: ovary	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E14.0 XX mesonephros	E14.5 (a)	TS22: mesonephros of female TS22: nephric duct of female TS22: paramesonephric duct of female	Present	.1-.4 µg; total RNA	C57BL/6JEi		Female
E14.0 XY gonad	E14.5 (a)	TS22: testis	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male
E14.0 XY mesonephros	E14.5 (a)	TS22: mesonephros of male TS22: nephric duct of male TS22: nephric duct of male, mesonephric portion	Present	.1-.4 µg; total RNA	C57BL/6JEi		Male

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression levels are higher in males than females.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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