Nf1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:30191 Gutmann DH, et al., Expression of the neurofibromatosis type 1 (NF1) gene during mouse embryonic development. Prog Brain Res. 1995;105:327-35
Assay type:	Western blot
MGI Accession ID:	MGI:3050535
Gene symbol:	Nf1
Gene name:	neurofibromin 1

Antibody:	G1
Detection system:	Secondary antibody coupled to alkaline phosphatase
Assay notes:	The antibody used for this assay recognizes multiple isoforms of this protein. The authors do not believe the lower band seen on the blot (at 220 kDa) is neurofibromin.

Results

Image: 1_neurofibromin

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	250 kDa	Genetic Background	Mutant Allele(s)	Sex
8	E8.0 (a)	TS12: embryo	Absent (b)	Not Specified		Not Specified
9	E9.0 (a)	TS14: embryo	Absent (b)	Not Specified		Not Specified
10	E10.0 (a)	TS16: embryo	Absent (b)	Not Specified		Not Specified
11	E11.0 (a)	TS18: embryo	Present	Not Specified		Not Specified
12	E12.0 (a)	TS20: embryo	Present	Not Specified		Not Specified
13	E13.0 (a)	TS21: embryo	Present	Not Specified		Not Specified
14	E14.0 (a)	TS22: embryo	Present	Not Specified		Not Specified
16	E16.0 (a)	TS24: embryo	Present	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The authors believe protein is present at this time but that levels fall below the level of detectability using this assay.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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