Gene Expression Data
Assay Details
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Assay
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Reference:
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J:82230
Hikichi T, et al., Imprinting regulation of the murine Meg1/Grb10 and human GRB10 genes; roles of brain-specific promoters and mouse-specific CTCF-binding sites. Nucleic Acids Res. 2003 Mar 1;31(5):1398-406
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:3045591
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Gene symbol:
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Grb10
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Gene name:
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growth factor receptor bound protein 10
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Probe:
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Meg1/Grb10 Polymorph
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Probe preparation:
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Double stranded DNA
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Visualized with:
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Autoradiography
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Assay notes:
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This experiment demonstrates that expression is Paternal in adult brain and maternal in adult kidney, liver and E9.5 embryo.
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Results
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Image: 1C
| Mol. Wt. Marker Lane |
| P adult
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TS28: mouse
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Not Applicable
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Present
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2 µg; total RNA |
C57BL/6 |
| Not Specified |
| P adult
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TS28: mouse
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Present
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Not Applicable
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2 µg; total RNA |
JF1 |
| Not Specified |
| P adult
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TS28: brain
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Present
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Weak
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2 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
| P adult
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TS28: brain
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Weak
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Present
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2 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
| P adult
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TS28: metanephros
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Absent
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Present
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2 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
| P adult
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TS28: metanephros
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Present
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Absent
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2 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
| P adult
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TS28: liver
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Absent
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Present
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2 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
| P adult
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TS28: liver
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Present
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Absent
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2 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
| E9.5
(c)
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TS15: embryo
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Absent
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Present
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2 µg; total RNA |
(C57BL/6 x JF1)F1 |
| Not Specified |
| E9.5
(c)
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TS15: embryo
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Present
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Absent
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2 µg; total RNA |
(JF1 x C57BL/6)F1 |
| Not Specified |
Notes:
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Analysis Tools