Htt RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:90356 Dixon KT, et al., Mouse Huntington's disease homolog mRNA levels: variation and allele effects. Gene Expr. 2004;11(5-6):221-31
Assay type:	RT-PCR
MGI Accession ID:	MGI:3043468
Gene symbol:	Htt
Gene name:	huntingtin

Probe:	Hdh-pE, Hdh-pF
Probe preparation:	Double stranded DNA
Assay notes:	Assay was done using the qRT-PCR system from Applied Biosystems. Data in Figure 2 shows variation in brain Hhd levels between 10 WT mice and 12 Hdh(CAG)150 mice.

Results

Image: 2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
1 WT	P adult	TS28: brain	Strong	5 µg; total RNA	C57BL/6J		Male
2 WT	P adult	TS28: brain	Strong	5 µg; total RNA	C57BL/6J		Male
3 WT	P adult	TS28: brain	Strong	5 µg; total RNA	C57BL/6J		Male
4 WT	P adult	TS28: brain	Strong	5 µg; total RNA	C57BL/6J		Male
5 WT	P adult	TS28: brain	Present	5 µg; total RNA	C57BL/6J		Male
6 WT	P adult	TS28: brain	Present	5 µg; total RNA	C57BL/6J		Male
7 WT	P adult	TS28: brain	Present	5 µg; total RNA	C57BL/6J		Male
8 WT	P adult	TS28: brain	Present	5 µg; total RNA	C57BL/6J		Male
9 WT	P adult	TS28: brain	Weak	5 µg; total RNA	C57BL/6J		Male
10 WT	P adult	TS28: brain	Weak	5 µg; total RNA	C57BL/6J		Male
1 (CAG)150	P adult	TS28: brain	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
2 (CAG)150	P adult	TS28: brain	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
3 (CAG)150	P adult	TS28: brain	Present	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
4 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
5 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
6 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
7 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
8 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
9 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
10 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
11 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male
12 (CAG)150	P adult	TS28: brain	Weak	5 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6J	Htt^tm2Detl/Htt^tm2Detl	Male

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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